p53 Antibody (2Q375): sc-71820
- p53 Antibody (2Q375) is a mouse monoclonal IgG1, cited in 11 publications, provided at 200 µg/ml
- raised against p53-beta galactosidase fusion protein
- recommended for detection of p53 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- See p53 (DO-1): sc-126 for p53 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p53 Antibody (2Q375). This work is in progress.
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p53 Antibody (2Q375), raised against the p53-beta galactosidase fusion protein, is a highly specific mouse monoclonal antibody for detecting p53 across species, including mouse, rat, and human. Recommended for use in western blot, immunoprecipitation, immunofluorescence, and Immunohistochemistry on paraffin-embedded sections, this antibody enables researchers to study p53 expression and function in various experimental assays. Given that p53 is a critical tumor suppressor, anti-p53 antibody (2Q375) is useful for cancer research and cellular studies. Encoded by the TP53 gene, p53 plays an essential role in maintaining genomic stability by regulating cellular processes such as DNA repair, cell cycle arrest, apoptosis, and senescence. In response to cellular stress or DNA damage, p53 halts the cell cycle at key checkpoints (G1/S or G2/M), allowing time for repair. If the damage is irreparable, p53 triggers apoptosis, eliminating genetically compromised cells and preventing tumor formation. Post-translational modifications, such as phosphorylation at serine residues (Ser6, Ser15, Ser46) by kinases like ATM, ATR, and Chk2, enhance p53’s stability and DNA-binding capacity. Acetylation by proteins such as p300 and CBP further stabilizes p53, while deacetylation by SIRT1 fine-tunes its activity in response to cellular aging and DNA damage. Antibodies targeting p53, like the p53 (2Q375) monoclonal antibody, are essential for studying the expression, post-translational modifications, and localization of p53 in cells and tissues. Santa Cruz Biotechnology’s p53 monoclonal antibodies allow researchers to investigate how p53 mutations, which are common in over half of cancers, disrupt its tumor suppressing function. Understanding p53’s role in disease mechanisms is critical for developing targeted therapies that restore p53 activity or compensate for its loss in tumor cells. By utilizing the anti-p53 antibody (2Q375), offers a deeper understanding into the molecular pathways regulated by p53 and how its dysfunction contributes to cancer. This knowledge is fundamental for advancing diagnostic strategies aimed at modulating the p53 pathway in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p53 Antibody (2Q375) References:
- Nitric oxide nitrates tyrosine residues of tumor-suppressor p53 protein in MCF-7 cells. | Chazotte-Aubert, L., et al. 2000. Biochem Biophys Res Commun. 267: 609-13. PMID: 10631110
- Tumor suppressor p53: regulation and function. | Somasundaram, K. 2000. Front Biosci. 5: D424-37. PMID: 10762600
- p53 expression in the normal murine eye. | Pokroy, R., et al. 2002. Invest Ophthalmol Vis Sci. 43: 1736-41. PMID: 12036973
- Accumulation of p53 protein in pterygia is not accompanied by TP53 gene mutation. | Schneider, BG., et al. 2006. Exp Eye Res. 82: 91-8. PMID: 16005868
- Loss of heterozygosity of the p53 gene and deregulated expression of its mRNA and protein in human brain tumors. | Rohini, K., et al. 2007. Mol Cell Biochem. 300: 101-11. PMID: 17180249
- Reversal of the DNA-binding-induced loop L1 conformational switch in an engineered human p53 protein. | Emamzadah, S., et al. 2014. J Mol Biol. 426: 936-44. PMID: 24374182
- Effect of a two-base insertion mutation of the TP53 gene on expression of p53 protein in canine histiocytic sarcoma cells. | Asada, H., et al. 2019. Am J Vet Res. 80: 680-688. PMID: 31246118
- Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance. | Bowden, AR., et al. 2020. Elife. 9: PMID: 32441252
- Analysis of the most representative tumour-derived p53 mutants reveals that changes in protein conformation are not correlated with loss of transactivation or inhibition of cell proliferation. | Ory, K., et al. 1994. EMBO J. 13: 3496-504. PMID: 8062826
- A cellular protein activates the sequence-specific DNA-binding of p53 by interacting with the central conserved region. | Srinivasan, R. and Maxwell, SA. 1996. Oncogene. 12: 193-200. PMID: 8552392
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p53 Antibody (2Q375) | sc-71820 | 200 µg/ml | $322.00 |