Anticorps p53 (2Q375) recommandé pour la détection de p53 d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps p53 (2Q375): sc-71820

Anticorps p53 (2Q375) est évalué 3.5 de 5 de 2.
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Fiches techniques

    • L'anticorps p53 2Q375 est un monoclonal IgG1 de souris, cité dans 10 publications, fourni en 200 µg/ml
    • raised against p53-beta galactosidase fusion protein
    • recommandé pour la détection de p53 d'origine mouse, rat et human par WB, IP, IF et IHC(P)
    • Voir p53 (DO-1): sc-126 pour d'autres anticorps conjugués p53, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p53 Antibody (2Q375). This work is in progress.
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Can sc-71820: p53 (2Q375) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: jenniferc
Thank you for your question. Yes, sc-71820: p53 (2Q375) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 4 de 5 de par Works well for WB in A Works well for WB in A-431 and mouse Lac Z whole cell lysates. -SCBT QC
Date de publication: 2015-01-06
Rated 3 de 5 de par Works in both mouse and human transfected lysates Works in both mouse and human transfected lysates. -SCBT QC
Date de publication: 2014-06-10
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