Date published: 2025-9-10
Visitez Santa Cruz Animal Health

00800 4573 8000

SCBT Portrait Logo
Seach Input
Contactez-nous
Se Connecter / S’inscrire Compte
Commande Rapide
Panier Articles (0)

Anticorps p-Ser/Phosphoserine (16B4): sc-81514

4.0(24)
Écrire une critiquePoser une question

Fiches techniques
  • L'anticorps p-Ser/Phosphoserine 16B4 est un monoclonal IgM κ L'Anticorps p-Ser/Phosphoserine, cité dans 95 publications, fourni en 50 µg/0.5 ml
  • élevé contre la phosphosérine
  • recommandé pour la détection de a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine d'origine mouse, rat, human et canine par WB et IP
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de p-Ser/Phosphoserine (16B4): sc-81514.
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire p-Ser/Phosphoserine (16B4). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

Produits de Support
Description
Fiche de Données et Protocoles
Informations Recherche

VOIR ÉGALEMENT...

Anticorps supplémentaires p-Ser incluant p-Thr et p-Tyr

p-Ser Antibody (16B4) is an IgM κ mouse monoclonal p-Ser antibody (also designated phosphorylated serine antibody, phospho serine antibody, or p-serine antibody) that detects the p-Ser protein of mouse, rat, human and canine origin by WB and IP. p-Ser Antibody (16B4) is available as the non-conjugated anti-p-Ser antibody. Protein kinases catalyze the phosphorylation of serine, threonine or tyrosine residues in target substrates, providing a mechanism of control for myriad cellular signaling pathways. Several families of kinases phosphorylate both serine and threonine residues in target substrates, including the Raf, Rsk, ROCK, PAK, Ak, and PKC families of serine/threonine protein kinases. The modification of proteins by phosphorylation can result in three dimensional changes to the structure of the protein and thereby alter its enzymatic activity or its ability to interact with other proteins. Antibodies targeted to phosphoserine may be used for the characterization of proteins with phosphorylated serine residues, and for the elucidation of cellular pathways involving serine phosphorylation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Anticorps p-Ser/Phosphoserine (16B4) Références:

  1. Structure primaire de la MEK, une protéine kinase qui phosphoryle le produit du gène ERK.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. La phosphosérine aminotransférase a un site actif conservé des microbes aux eucaryotes supérieurs avec des déviations mineures.  |  Singh, RK., et al. 2021. Protein Pept Lett. 28: 996-1008. PMID: 33588715
  3. La régulation à la baisse de la phosphosérine phosphatase potentialise les environnements immunitaires tumoraux afin d'améliorer la thérapie de blocage du point de contrôle immunitaire.  |  Peng, ZP., et al. 2023. J Immunother Cancer. 11: PMID: 36849198
  4. Les membranes de chitosane chargées de phosphosérine favorisent la régénération osseuse en activant les cellules souches endogènes.  |  Ke, Y., et al. 2023. Front Bioeng Biotechnol. 11: 1096532. PMID: 37034248
  5. Biosynthèse et codage génétique de la phosphosérine non hydrolysable dans des protéines recombinantes chez Escherichia coli.  |  Zhu, P., et al. 2023. Bio Protoc. 13: e4861. PMID: 37969748
  6. L'excès de phosphosérine-129 α-synucléine induit un trafic de vésicules synaptiques et des défauts de déclustering dans une synapse de vertébré.  |  Wallace, JN., et al. 2024. Mol Biol Cell. 35: ar10. PMID: 37991902
  7. Voies de transduction du signal MAP-kinase humaines indépendantes définies par les isoformes MEK et MKK.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  8. Mutant constitutif et site de phosphorylation de sérine régulateur putatif de la MAP kinase kinase de mammifère (MEK1).  |  Pagès, G., et al. 1994. EMBO J. 13: 3003-10. PMID: 8039496
  9. Phosphorylation du domaine de transrépression de c-Fos par la protéine kinase activée par les mitogènes et la ribosomale S6 kinase 90-kDa.  |  Chen, RH., et al. 1993. Proc Natl Acad Sci U S A. 90: 10952-6. PMID: 8248197
  10. ROCK-I et ROCK-II, deux isoformes de la protéine serine/thréonine kinase Rho-associée formant un enroulement en spirale chez la souris.  |  Nakagawa, O., et al. 1996. FEBS Lett. 392: 189-93. PMID: 8772201
  11. L'homologue humain de Ste20, hPAK1, relie les GTPases à la voie de la MAP kinase JNK.  |  Brown, JL., et al. 1996. Curr Biol. 6: 598-605. PMID: 8805275
  12. Nouveaux inhibiteurs de la phosphosérine phosphatase.  |  Hawkinson, JE., et al. 1997. Eur J Pharmacol. 337: 315-24. PMID: 9430431

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps p-Ser/Phosphoserine (16B4)

sc-81514
50 µg/0.5 ml
$316.00

Is there any possibility that p-Ser Antibody (16B4): sc-81514 does work for yeast samples as well...?

Posée par: yolyol
Thank you for your question. This antibody has only been tested in samples of mouse, rat, human and canine origin. It has not been tested in yeast species.
Répondue par: Tech Support Europe
Date de publication: 2021-02-26

Hello, Which buffer is better to detect p-Ser antibody, skim-milk or 3-5% BSA? I immunoprecipitated phospho-protein and used 3% BSA in TBS-T buffer for blocking and 1st antibody but I couldn't get specific phosphorylation band.

Posée par: chohee0720
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Répondue par: Tech Support Europe
Date de publication: 2022-07-16

HI , I recently purchased this pserine antibody and I do see a band at 20kDa. why do i see a band arounf 20kda for pSerine?

Posée par: Dhanusha
Thank you for your question. As p-Ser Antibody (16B4): sc-81514 detects a broad range of serine-phosphorylated proteins in crude cell extracts, a signal at 20 kDa can respond to a large variety of different proteins of this molecular weight. The identity of this protein could be determined by assays such as MALDI-TOF.
Répondue par: Tech Support Europe
Date de publication: 2020-07-14

The p-Ser Antibody (16B4) antibody is a monoclonal mouse IgM κ (kappa light chain). Still, you recommend the m-IgGκ BP-HRP: sc-516102 as a secondary antibody. But this a IgG-kappa specific ab. Would it still work?

Posée par: PeterPM
Thank you for your question. Yes, m-IgGκ BP-HRP: sc-516102 can detect any antibody that possesses a kappa light chain, including p-Ser Antibody (16B4): sc-81514, other IgM, and IgA antibodies.
Répondue par: Technical Support Europe
Date de publication: 2019-05-06

Just wondering if your products of sc-81516 and sc-81514 recognize any of the phosphorylated S/T/Y, or they require certain residues beside these phosphorylated residues (like a certain motif).

Posée par: Zhyuhao
Thank you for your question. These antibodies both detect a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Additional information regarding the detected residues is not available.
Répondue par: Tech Service
Date de publication: 2019-04-10

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Posée par: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Répondue par: Tech Service
Date de publication: 2018-08-09

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Posée par: Constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Répondue par: Technical Support
Date de publication: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Posée par: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Répondue par: Technical Support
Date de publication: 2016-12-22
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_8
  • loc_fr_FR, sid_81514, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 119ms
  • QUESTIONS, PRODUCT
Rated 4 de 5 de par p-Ser (16B4): sc-81514Western blot: Detecta multiples proteínas en extractos totales de células CRFK con 20 ug de proteína a una dilución de 1:500 y 1:1000 IP: Se utilizó 5 y 7 uL para inmunoprecipitar proteínas fosforiladas de células CRFK. Se utilizó la dilución de 1:500 para identificar las proteínas inmunoprecipitadas y sí se observan diversas bandas con diferentes pesos moleculares
Date de publication: 2024-04-01
Rated 5 de 5 de par Works greatIt adequately detects phosphoserine antibodies. Even though it has some background, you can clean it up by more TBS T20 washes.
Date de publication: 2024-03-28
Rated 1 de 5 de par 使えなかった一切バンドがでなかった。通常リン酸化抗体はブロードなバンドがみえるが、ひとつもバンドがみえなかった。
Date de publication: 2020-11-18
Rated 3 de 5 de par Pretty good antibodyThe antibody works pretty well upon immunoprecipitation of the phosphorylated protein. On total cellular extract is too weak.
Date de publication: 2020-10-07
Rated 3 de 5 de par Worked in WBphosphorylation was detected in Pull downed protein in WB
Date de publication: 2019-07-23
Rated 5 de 5 de par Successful detectionSuccessful detection of Ser phosphorylation in immunoprecipitates
Date de publication: 2018-12-07
Rated 3 de 5 de par P-Ser AntibodyCompared to other p-ser abs there was a lower background. Signal was much lower too. Tested in HeLa total lysates.
Date de publication: 2018-02-14
Rated 3 de 5 de par Nice antibodyWe used this antibody for IP last month. It works well for some primary proteins. Overall a decent buy.
Date de publication: 2017-11-13
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_24
  • loc_fr_FR, sid_81514, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
Anticorps p-Ser/Phosphoserine (16B4) est évalué 4.0 de 5 de 24.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_24
  • loc_fr_FR, sid_81514, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 116ms
  • REVIEWS, PRODUCT
00800 4573 8000
Visitez Santa Cruz Animal Health|Visitez San Juan Ranch
Santa Cruz Biotechnology, Inc. est leader mondial dans le développement de produits pour la recherche biomédicale. Contactez-nous au 1-800-457-3801.
Copyright © 2007-2025 Santa Cruz Biotechnology, Inc. Tous Droits Réservés. “Santa Cruz Biotechnology”, et le logo Santa Cruz Biotechnology, Inc., “Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, le logo San Juan Ranch, “UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, et “EZ Touch” sont des marques déposées de Santa Cruz Biotechnology, Inc. Toutes les marques sont la propriété de leurs propriétaires respectifs.