Date published: 2025-9-10

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Anticorps p-eNOS (pS633.37): sc-136198

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Fiches techniques
  • L'Anticorps p-eNOS (pS633.37) est un monoclonal de souris IgG1 (chaîne légère kappa) fourni en 200 µg/ml
  • opposé à une courte séquence d'acides aminés contenant la Ser 633 phosphorylée de NOS3 d'origine human.
  • recommandé pour la détection de Ser 633 phosphorylated NOS3 d'originie human par WBIP
  • Le m-IgGκ BP-HRP est le réactif de détection secondaire préféré pour l'anticorps p-eNOS (pS633.37) pour les applications WB. Ce réactif est désormais proposé en association avec l'anticorps p-eNOS (pS633.37)(voir les informations relatives à la commande ci-dessous). Pour d'autres conjugués m-IgGκ BP, voir notre liste complète de protéines de liaison à l'IgG de souris.

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    Nitric oxide (NO), produced by the endothelial NO synthase (NOS3), is a fundamental determinant of cardiovascular homeostasis that maintains system blood pressure, vascular remodeling and angiogenesis. NOS3 is stimulated, in a phosphatidylinositol 3-kinase (PI 3-kinase)-dependent fashion, by treatment of endothelial cells with insulin-like growth factor-1 and vascular endothelial growth factor (VEGF). The serine/ threonine protein kinase Akt/PKB is an important downstream target of PI 3-kinase, regulating VEGF-stimulated endothelial cell survival. NOS3 activation via phosphorylation of serine 1177 by Akt/PKB is necessary and sufficient for VEGF-mediated endothelial cell migration. Therefore, Akt/PKB can directly phosphorylate NOS3 on serine 1177, activating the enzyme and leading to NO production.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps p-eNOS (pS633.37)

    sc-136198
    200 µg/ml
    $316.00

    p-eNOS (pS633.37): m-IgGκ BP-HRP Kit

    sc-521342
    200 µg Ab, 40 µg BP
    $354.00

    Hi, I would like to know if this antibody have been tested for bovine samples?  Thank you very much

    Posée par: Anonyme
    Thank you for your question. Anti-p-NOS3 Antibody (pS633.37): sc-136198 is only recommended for detection of Ser 633 phosphorylated NOS3 of human origin by WB IP. We do not have any data that would indicate compatibility with bovine samples.
    Répondue par: Tech Support Europe
    Date de publication: 2021-12-22

    How much sample do you recommend loading on the gel for Western blot detection using p-NOS3 (pS633.37): sc-136198?

    Posée par: Randy McDonald
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Répondue par: Technical Support
    Date de publication: 2017-03-27
    • y_2025, m_8, d_27, h_4CST
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    Rated 4 de 5 de par Good results by Western blot analysis ofGood results by Western blot analysis of NOS3 phosphorylation in human endothelial cells either untreated (A) or treated (B) with lambda phosphatase.
    Date de publication: 2014-04-14
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    Anticorps p-eNOS (pS633.37) est évalué 4.0 de 5 de 1.
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