Ox40L Antibody (OX89): sc-53105

Ox40L Antibody (OX89) is a rat monoclonal IgG1 antibody that detects Ox40L of mouse origin by flow cytometry (FCM). Ox40L (OX89) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. The Ox40 ligand, also known as gp34, is a type II membrane protein characterized by its lack of signal peptide, which is crucial for function in immune responses. As a member of the tumor necrosis factor (TNF) superfamily, Ox40L serves as a vital costimulatory molecule that facilitates interactions between dendritic cells and T cells, as well as promoting T cell homing and B cell activation. The engagement of Ox40L with Ox40 provides a potent costimulatory signal to effector T cells, significantly enhancing activation and proliferation. Notably, Ox40L is predominantly expressed on activated B cells, while Ox40 is found on activated T cells, underscoring the importance of this interaction in regulating antigen-specific T cell responses in vivo. This dynamic is particularly critical during both the priming and effector phases of T cell activation, where Ox40L is expressed on antigen-presenting cells (APCs), thereby playing a key role in orchestrating effective immune responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ox40L Antibody (OX89) References:

1. OX40-OX40 ligand interaction in T-cell-mediated immunity and immunopathology.  |  Ishii, N., et al. 2010. Adv Immunol. 105: 63-98. PMID: 20510730
2. OX40/OX40 ligand interactions in T-cell regulation and asthma.  |  Kaur, D. and Brightling, C. 2012. Chest. 141: 494-499. PMID: 22315115
3. Roles of OX40 and OX40 Ligand in Mycosis Fungoides and Sézary Syndrome.  |  Kawana, Y., et al. 2021. Int J Mol Sci. 22: PMID: 34830466
4. OX40 Ligand-Mannose-Binding Lectin Fusion Protein Induces Potent OX40 Cosignaling in CD4+ T Cells.  |  Sato, A., et al. 2022. Biol Pharm Bull. 45: 1798-1804. PMID: 36450532
5. Safety and efficacy of amlitelimab, a fully human nondepleting, noncytotoxic anti-OX40 ligand monoclonal antibody, in atopic dermatitis: results of a phase IIa randomized placebo-controlled trial.  |  Weidinger, S., et al. 2023. Br J Dermatol. 189: 531-539. PMID: 37463508
6. OX40/OX40 ligand and its role in precision immune oncology.  |  Thapa, B., et al. 2024. Cancer Metastasis Rev. 43: 1001-1013. PMID: 38526805
7. The role of OX40 ligand/OX40 axis signalling in atopic dermatitis.  |  Guttman-Yassky, E., et al. 2024. Br J Dermatol. 191: 488-496. PMID: 38836560
8. Phase 2b randomized clinical trial of amlitelimab, an anti-OX40 ligand antibody, in patients with moderate-to-severe atopic dermatitis.  |  Weidinger, S., et al. 2024. J Allergy Clin Immunol.. PMID: 39522654
9. Generation and characterization of OX40-ligand fusion protein that agonizes OX40 on T-Lymphocytes.  |  Sato, A., et al. 2024. Front Immunol. 15: 1473815. PMID: 39867912
10. OX40 ligand promotes follicular helper T cell differentiation and development in mice with immune thrombocytopenia.  |  Yang, Z., et al. 2025. J Zhejiang Univ Sci B. 26: 240-253. PMID: 40082203

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ox40L Antibody (OX89) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ox40L Antibody (OX89) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ox40L Antibody (OX89) Specifications

Alternate Product Name: Ox40L

Product Clone Name: OX89

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Ox40L

Epitope Species: rat and mouse

Product Reactivity/Detection: Ox40L

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: FCM

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ox40L Antibody (OX89): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Ox40L antibody is Ox40L Antibody (RR19): sc-80271.