OB-cadherin Double Nickase Plasmid (h): sc-401051-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OB-cadherin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OB-cadherin Double Nickase Plasmid (h) and OB-cadherin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDH11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OB-cadherin Antibody (F-3): sc-365867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OB-cadherin Double Nickase Plasmid (h) | sc-401051-NIC | 20 µg | $410.00 | |||
OB-cadherin Double Nickase Plasmid (h2) | sc-401051-NIC-2 | 20 µg | $410.00 |
CDH11 encodes OB-cadherin, a calcium-dependent cell–cell adhesion molecule enriched in mesenchymal lineages that helps organize adherens junctions and coordinate tissue architecture. Through interactions with catenins and coupling to the actin cytoskeleton, OB-cadherin influences contact-mediated signaling, cell polarity, and migratory behavior during development and stromal remodeling. CDH11 activity intersects with pathways governing epithelial–mesenchymal transition, extracellular matrix organization, and mechanotransduction that shape fibroblast and osteoblast function. Dysregulated CDH11 expression has been associated with fibrotic and inflammatory microenvironments and is frequently studied in the context of tumor stroma dynamics and osteogenic differentiation programs.
OB-cadherin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDH11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDH11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDH11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDH11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.