NRF-1 CRISPR/Cas9 KO Plasmid (m): sc-421961

Overview

Mouse Nrf1 (NRF-1) encodes a transcription factor that coordinates nuclear control of mitochondrial biogenesis and respiratory function by regulating genes involved in oxidative phosphorylation, mitochondrial DNA transcription/replication machinery, and cellular energy metabolism. NRF-1 integrates nutrient and growth factor signaling with cell-cycle progression and redox homeostasis, supporting proteostasis and adaptive responses to metabolic stress. Dysregulation of NRF-1–dependent transcriptional programs has been linked to altered mitochondrial function and oxidative stress phenotypes relevant to neurodegeneration, cardiometabolic dysfunction, and cancer-associated metabolic reprogramming in experimental models. As a central node in mitochondrial–nuclear crosstalk, NRF-1 is frequently studied in pathways governing bioenergetics, ROS handling, and transcriptional networks that shape differentiation and stress resilience.

NRF-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nrf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nrf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nrf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NRF-1 protein expression.

This CRISPR knockout system enables efficient generation of Nrf1-deficient cell models for investigation of NRF-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Nrf1 exon(s) critical for NRF-1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Nrf1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by NRF-1 CRISPR/Cas9 KO Plasmid (m) and NRF-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nrf1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by NRF-1 HDR Plasmid (m) and NRF-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nrf1 homology arms to support homology-directed repair at defined Nrf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.