Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NPC1L1 CRISPR/Cas9 KO Plasmid (h): sc-401741
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPC1L1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPC1L1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NPC1L1 Antibody (G-1): sc-166802
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPC1L1 CRISPR/Cas9 KO Plasmid (h) | sc-401741 | 20 µg | $397.00 |
Overview
NPC1L1 (Niemann-Pick C1-like 1) is a multi-pass membrane protein that mediates cellular uptake of dietary and biliary cholesterol and other sterols, functioning at the plasma membrane and within endocytic trafficking compartments. It participates in sterol sensing and vesicular transport processes that influence intracellular lipid homeostasis and lipoprotein handling. NPC1L1 activity intersects with pathways controlling cholesterol absorption, membrane composition, and lipid-dependent signaling. Dysregulation of NPC1L1-associated sterol transport is relevant to metabolic phenotypes linked to altered cholesterol balance and lipid-driven inflammatory states.
NPC1L1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NPC1L1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NPC1L1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NPC1L1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPC1L1 protein expression.
This CRISPR knockout system enables efficient generation of NPC1L1-deficient cell models for investigation of NPC1L1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NPC1L1 exon(s) critical for NPC1L1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NPC1L1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPC1L1 CRISPR/Cas9 KO Plasmid (h) and NPC1L1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NPC1L1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPC1L1 HDR Plasmid (h) and NPC1L1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NPC1L1 homology arms to support homology-directed repair at defined NPC1L1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.