Date published: 2025-9-7

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Anticorps Nogo (C-4): sc-271878

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Fiches techniques
  • L'anticorps Nogo C-4 est un monoclonal IgM κ L'Anticorps Nogo, cité dans 27 publications, fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 18-41 au niveau N-terminus de Nogo d'origine human
  • recommandé pour la détection de Nogo A, Nogo B and foocen d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont porcine
  • disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; à la phycoérythrine, à la fluorescéine, à l'Alexa Fluor® 488 ou à l'Alexa Fluor® 647 pour IF, IHC(P) et FCM
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Nogo (C-4): sc-271878.
  • Le m-IgGκ BP-HRP est le réactif de détection secondaire préféré pour l'anticorps Nogo (C-4) pour les applications WB et IHC(P). Ce réactif est désormais proposé en association avec l'anticorps Nogo (C-4)(voir les informations relatives à la commande ci-dessous). Pour d'autres conjugués m-IgGκ BP, voir notre liste complète de protéines de liaison à l'IgG de souris.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Nogo Antibody (C-4) is an IgM κ mouse monoclonal Nogo antibody (also designated neurite outgrowth inhibitor (Nogo) antibody, or neuroendocrine secretion regulating protein antibody) that detects the Nogo protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Nogo Antibody (C-4) is available as both the non-conjugated anti-Nogo antibody form, as well as multiple conjugated forms of anti-Nogo antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. CNS white matter is selectively inhibitory for axonal out-growth. Nogo (also designated NI250 and Reticulon 4-A) is an oligodendrocyte-specific member of the Reticulon family and is a component of CNS white matter that inhibits axon outgrowth, induces collapse of growth cones of chick dorsal root ganglion cells, and inhibits the spreading of 3T3 fibroblasts. Other members of the reticulin protein family do not inhibit axon extension and are thought to have a role in ER function. Nogo is expressed by oligodendrocytes but not by Schwann cells, and associates primarily with the endoplasmic reticulum. Nogo has three different splice forms, Nogo-A, Nogo-B and Nogo-C.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Anticorps Nogo (C-4) Références:

  1. Identification de l'inhibiteur Nogo de la régénération axonale en tant que protéine Reticulon.  |  GrandPré, T., et al. 2000. Nature. 403: 439-44. PMID: 10667797
  2. Fonction du récepteur Nogo-A/Nogo-A dans la maladie d'Alzheimer.  |  Xu, YQ., et al. 2015. CNS Neurosci Ther. 21: 479-85. PMID: 25732725
  3. Anticorps Nogo-A pour la sclérose en plaques progressive.  |  Ineichen, BV., et al. 2017. CNS Drugs. 31: 187-198. PMID: 28105588
  4. La liaison du RTN4/NoGo-récepteur aux GPCRs d'adhésion BAI régule le développement neuronal.  |  Wang, J., et al. 2021. Cell. 184: 5869-5885.e25. PMID: 34758294
  5. La liaison des récepteurs RTN4/NoGo- aux GPCRs d'adhésion BAI régule le développement neuronal.  |  Wang, J., et al. 2022. Cell. 185: 218. PMID: 34995515

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Nogo (C-4)

sc-271878
200 µg/ml
$316.00

Nogo (C-4): m-IgGκ BP-HRP Kit

sc-522088
200 µg Ab, 40 µg BP
$354.00

Anticorps Nogo (C-4) AC

sc-271878 AC
500 µg/ml, 25% agarose
$416.00

Anticorps Nogo (C-4) Alexa Fluor® 488

sc-271878 AF488
200 µg/ml
$357.00

Anticorps Nogo (C-4) Alexa Fluor® 647

sc-271878 AF647
200 µg/ml
$357.00

Anticorps Nogo (C-4) FITC

sc-271878 FITC
200 µg/ml
$330.00

Anticorps Nogo (C-4) HRP

sc-271878 HRP
200 µg/ml
$316.00

Nogo (C-4) peptide neutralisant

sc-271878 P
100 µg/0.5 ml
$68.00

Anticorps Nogo (C-4) PE

sc-271878 PE
200 µg/ml
$343.00

What region of Nogo-A, B, and C does this antibody recognize? I have a KO mouse with a flxed region of Exon 4/5 ( first two common exons between all Nogo proteins). I am still seeing Nogo expression in cells where Nogo should be KO. Confirmed KO via PCR 

Posée par: elixir_w
Thank you for your question. Nogo Antibody (C-4): sc-271878 is specific for an epitope mapping between amino acids 18-41 at the N-terminus of Nogo of human origin.
Répondue par: Tech Support Europe
Date de publication: 2022-11-30

The sample blot shows a protein of about 55 kDa, corresponding to the Nogo B isoform, but no band around 125 kDa, the molecular weight of the Nogo A isoform. Is this antibody preference or differential expression of the isoforms in HEK cells?

Posée par: shoran
Thank you for your question. The Western blot data shown on our website is representative of what you can expect when using this antibody. We haven't tested for differential isoform expression in HEK cells. There is Western blot data in Neuro-2A and EOC20 whole cell lysates showing a signal detected around 220 kDa, the expected observed molecular weight for Nogo A.
Répondue par: Technical Support
Date de publication: 2019-02-25

What is the Molecular Weight of the Nogo Antibody (C-4)?

Posée par: asj64
We do not determine the exact molecular weight for each individual antibody. However, the expected molecular weight detected by this Nogo Antibody (C-4): sc-271878 can be found on our product datasheet. A link is provided below. https://datasheets.scbt.com/sc-271878.pdf
Répondue par: Technical Support
Date de publication: 2018-01-10

What application is the blocking peptide sc-271878 P appropriate for?

Posée par: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par very strong band!I bought this antibody two weeks for WB, then I got a strong band.
Date de publication: 2019-07-16
Rated 5 de 5 de par Great Antibody!I tested 30-32 antibodies from Santa Cruz. I used the Nogo Antibody (C-4) to detect RTN4 from mouse brain lysates. I detected a single band in WB (1:200).
Date de publication: 2018-07-05
Rated 5 de 5 de par Excellent for NogoA & B, but not for NoGo CIt's an excellent antibody for detection of NogoA and NogoB in western blot and IF. I used 1:60 dilution of this antibody in IF, for staining Nogo A & B in PFA fixed HeLa cells. I used 1:200 dilution in western blot for detecting NogoA & B in HeLa cell lysate. There was a strong band for NogoB and a faint band for NogoA, but no band for NogoC.
Date de publication: 2018-02-23
Rated 5 de 5 de par RecommendedNogo Antibody are involved in some advantages, such as high specificity, sensitivity, convenience, low cost and short cycle.
Date de publication: 2017-10-18
Rated 5 de 5 de par very good antibodyI have tried twoNogo A antibodies of the other companies for several times, but the results are disappointed. This one is good, you can easily get strong and clear bands with this antibody.
Date de publication: 2017-09-27
Rated 5 de 5 de par Great antibodyWorked well for IF staining of retinal cross sections from mouse.
Date de publication: 2017-09-18
Rated 3 de 5 de par Not usable on J774A.1 cellsProduces only very dim diffuse staining of J774A.1 cells. On the other hand, it was a free sample, so no harm done.
Date de publication: 2017-05-11
Rated 5 de 5 de par Produced publishable WB data in human and ratThis Nogo monoclonal antibody was referenced in the literature for the use of WB on human and mouse samples
Date de publication: 2017-02-09
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Anticorps Nogo (C-4) est évalué 4.8 de 5 de 11.
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