Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NMD3 CRISPR/Cas9 KO Plasmid (h): sc-406373
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMD3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NMD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMD3 Antibody (A-5): sc-515426
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMD3 CRISPR/Cas9 KO Plasmid (h) | sc-406373 | 20 µg | $397.00 |
Overview
NMD3 encodes an essential ribosome biogenesis factor that functions as an adaptor for the export of pre-60S ribosomal subunits from the nucleus to the cytoplasm. By coordinating with the CRM1/XPO1 export pathway and remodeling events during late 60S maturation, NMD3 helps ensure productive assembly of translationally competent ribosomes and maintenance of proteostasis. Disruption of NMD3-dependent ribosome export can trigger nucleolar stress responses and alter cell-cycle progression through downstream effects on global translation. Because ribosome biogenesis is tightly linked to growth control, aberrant regulation of this process is relevant to studies of proliferative and stress-associated disease mechanisms, including ribosomopathies and cancer-associated translational rewiring.
NMD3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NMD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NMD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NMD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NMD3 protein expression.
This CRISPR knockout system enables efficient generation of NMD3-deficient cell models for investigation of NMD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NMD3 exon(s) critical for NMD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NMD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NMD3 CRISPR/Cas9 KO Plasmid (h) and NMD3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NMD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NMD3 HDR Plasmid (h) and NMD3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NMD3 homology arms to support homology-directed repair at defined NMD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.