neurolysin Antibody (B-3): sc-398420
- neurolysin Antibody (B-3) is a mouse monoclonal IgG1 κ neurolysin antibody provided at 200 µg/ml
- raised against amino acids 644-704 mapping at the C-terminus of neurolysin of human origin
- neurolysin Antibody (B-3) is recommended for detection of neurolysin of human origin by WB, IP, IF and ELISA
- Anti-neurolysin Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for neurolysin Antibody (B-3) for WB applications. These reagents are now offered in bundles with neurolysin Antibody (B-3) (see ordering information below).
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SEE ALSO...
neurolysin Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects neurolysin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-neurolysin antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neurolysin, a zinc-dependent metallopeptidase, plays a crucial role in the degradation of neuropeptides and small peptide neurotransmitters, thereby influencing numerous biological processes such as neurohormonal signaling and neurotransmission. The unique structure of neurolysin′s active site, featuring extended connecting loop regions within a five-stranded beta-sheet and two active site helices, enables cleavage of a wide range of peptide sequences. This structural adaptability allows neurolysin to effectively modulate the activity of various neuropeptides, making neurolysin essential for maintaining homeostasis in the nervous system. Neurolysin′s predominant localization to neuronal perikarya in the brain highlights neurolysin′s significance in central nervous system function and potential implications in neurodegenerative diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
neurolysin Antibody (B-3) References:
- Differential subcellular distribution of neurolysin (EC 3.4.24.16) and thimet oligopeptidase (EC 3.4.24.15) in the rat brain. | Massarelli, EE., et al. 1999. Brain Res. 851: 261-5. PMID: 10642854
- Crystallization and preliminary analysis of neurolysin. | Lian, W., et al. 2000. Acta Crystallogr D Biol Crystallogr. 56: 1644-6. PMID: 11092934
- Structure of neurolysin reveals a deep channel that limits substrate access. | Brown, CK., et al. 2001. Proc Natl Acad Sci U S A. 98: 3127-32. PMID: 11248043
- Oligopeptidases, and the emergence of the prolyl oligopeptidase family. | Barrett, AJ. and Rawlings, ND. 1992. Biol Chem Hoppe Seyler. 373: 353-60. PMID: 1515061
- Characterization of a mitochondrial metallopeptidase reveals neurolysin as a homologue of thimet oligopeptidase. | Serizawa, A., et al. 1995. J Biol Chem. 270: 2092-8. PMID: 7836437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neurolysin Antibody (B-3) | sc-398420 | 200 µg/ml | $322.00 | |||
neurolysin Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-530788 | 200 µg Ab; 10 µg BP | $361.00 | |||
neurolysin Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-524331 | 200 µg Ab, 40 µg BP | $361.00 | |||
neurolysin Antibody (B-3): m-IgG1 BP-HRP Bundle | sc-544252 | 200 µg Ab; 20 µg BP | $361.00 | |||
neurolysin Antibody (B-3) AC | sc-398420 AC | 500 µg/ml, 25% agarose | $424.00 | |||
neurolysin Antibody (B-3) HRP | sc-398420 HRP | 200 µg/ml | $322.00 | |||
neurolysin Antibody (B-3) FITC | sc-398420 FITC | 200 µg/ml | $336.00 | |||
neurolysin Antibody (B-3) PE | sc-398420 PE | 200 µg/ml | $349.00 | |||
neurolysin Antibody (B-3) Alexa Fluor® 488 | sc-398420 AF488 | 200 µg/ml | $364.00 | |||
neurolysin Antibody (B-3) Alexa Fluor® 546 | sc-398420 AF546 | 200 µg/ml | $364.00 | |||
neurolysin Antibody (B-3) Alexa Fluor® 594 | sc-398420 AF594 | 200 µg/ml | $364.00 | |||
neurolysin Antibody (B-3) Alexa Fluor® 647 | sc-398420 AF647 | 200 µg/ml | $364.00 | |||
neurolysin Antibody (B-3) Alexa Fluor® 680 | sc-398420 AF680 | 200 µg/ml | $364.00 | |||
neurolysin Antibody (B-3) Alexa Fluor® 790 | sc-398420 AF790 | 200 µg/ml | $364.00 |