Date published: 2026-10-17
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alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) - Western Blotting - Image 376749
Na+/K+-ATPase α1抗体 (F-2): sc-514614. HeLa (A), Jurkat (B) 和 SK-MEL-24 (C) 全细胞裂解液中 Na+/K+-ATPase α1 表达的 Western 印迹分析.
Na+/K+-ATPase α1抗体 (F-2): sc-514614. HeLa (A), Jurkat (B) 和 SK-MEL-24 (C) 全细胞裂解液中 Na+/K+-ATPase α1 表达的 Western 印迹分析.

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2): sc-514614

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说明书
  • alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 F-2 是小鼠单克隆 IgG1 κ，alpha 1 Sodium Potassium ATPase/ATP1A1抗体, 在18篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Na+/K+-ATPase α1的N-terminus附近的氨基酸54-76之间
  • alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat, human 和monkey 来源的 Na+/K+-ATPase α1
  • 抗alpha 1 Sodium Potassium ATPase/ATP1A1抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) 打包提供（请参阅下面的订购信息）。
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附加Sodium/Potassium-ATPase Protein抗体，包括H+/K+ ATPase β, Na+/K+-ATPase α, Na+/K+-ATPase β 1, Na+/K+-ATPase β 3和Na+/K+-ATPase α 1

Na+/K+-ATPase α1 抗体（F-2）是一种小鼠单克隆 IgG1 kappa 轻链抗体，专门用于识别人类 Na+/K+-ATPase α1 亚基（也称为钠钾泵）。Na+/K+-ATPase α1 单克隆抗体（F-2）针对靠近 N 端 54-76 氨基酸之间的表位，确保在各种应用中准确检测蛋白质。Na+/K+-ATPase α1是一种重要的膜结合酶，位于质膜上，负责通过ATP水解将三个Na⁺离子主动运出细胞，将两个K⁺离子运入细胞，从而维持钠离子和钾离子的电化学梯度。这种蛋白质会经历重要的翻译后修饰，包括糖基化，这对于正确的折叠、稳定性、膜定位和功能活性至关重要。β亚基的糖基化对于α1亚基的正确组装和插入质膜尤为重要，而磷酸化事件则调节其活性及其与其他细胞蛋白的相互作用。Na+/K+-ATPase α1 (F-2) 抗体推荐用于免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA），可广泛地与来自小鼠、大鼠、人类和猴子的 Na+/K+-ATPase α1 以及包括绵羊、猪和犬在内的其他物种的 Na+/K+-ATPase α1 发生反应。这种广泛的物种反应性使得Na+/K+-ATPase α1（F-2）单克隆抗体成为研究人员研究离子转运机制、细胞稳态和相关病理生理状况的宝贵工具。了解Na+/K+-ATPase α1的翻译后修饰对于阐明其在维持细胞功能中的作用以及参与高血压、心脏功能障碍和神经系统疾病等疾病至关重要。Na+/K+-ATPase α1 (F-2)抗体是推进对这种关键膜蛋白的复杂调节和功能进行科学研究的必要试剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2)

sc-514614
200 µg/ml
$322.00

alpha 1 Sodium Potassium ATPase/ATP1A1 (F-2): m-IgG Fc BP-HRP 套装

sc-527922
200 µg Ab; 10 µg BP
$361.00

alpha 1 Sodium Potassium ATPase/ATP1A1 (F-2): m-IgG1 BP-HRP 套装

sc-533295
200 µg Ab; 20 µg BP
$361.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) AC

sc-514614 AC
500 µg/ml, 25% agarose
$424.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) HRP

sc-514614 HRP
200 µg/ml
$322.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) FITC

sc-514614 FITC
200 µg/ml
$336.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) PE

sc-514614 PE
200 µg/ml
$349.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) Alexa Fluor® 488

sc-514614 AF488
200 µg/ml
$364.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) Alexa Fluor® 546

sc-514614 AF546
200 µg/ml
$364.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) Alexa Fluor® 594

sc-514614 AF594
200 µg/ml
$364.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) Alexa Fluor® 647

sc-514614 AF647
200 µg/ml
$364.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) Alexa Fluor® 680

sc-514614 AF680
200 µg/ml
$364.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) Alexa Fluor® 790

sc-514614 AF790
200 µg/ml
$364.00

alpha 1 Sodium Potassium ATPase/ATP1A1 (F-2) 中和勝肽

sc-514614 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-514614 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by Dokinson from Good AntibodyUsed with 40 ug of protein extract from bronchial epithelial cells and worked well, it needs long exposure but the result is very clean and specific.
Date published: 2021-04-22
Rated 5 out of 5 by Arnaud from Works Great by immunofluorescence.Worked at 1:500 on rat brain tissue formalin fixed and paraffin embedded. Great product.
Date published: 2020-11-30
Rated 5 out of 5 by Fmickey from Very works with western blottingThis antibody very works with western blotting with SAS cell lysate.
Date published: 2020-06-12
Rated 5 out of 5 by Markopolo from This antibody works greatVery specific neuronal membrane stain. IgG3 so you can combine with many other antibodies!
Date published: 2019-01-24
Rated 1 out of 5 by Asifuga from Didn't worked for western blottingDidn't worked for western blotting when used for detecting membrane proteins from MCF-7, Jurkat and PC-3 cells
Date published: 2019-01-02
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alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (F-2) is rated 4.0 out of 5 by 5.
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