N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride (CAS 911-77-3)
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N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride (BAPNA) is a synthetic peptide used extensively in biochemical assays to investigate protease activity, specifically serine proteases. This compound serves as a chromogenic substrate; upon cleavage by the protease, it releases 4-nitroaniline, a molecule that can be detected spectrophotometrically due to its yellow color. The rate of color change is directly proportional to the enzyme activity, allowing for the quantitative analysis of protease activity in various research applications.N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride′s design incorporates the arginine residue, making it a selective substrate for trypsin-like serine proteases. This specificity is for elucidating mechanisms of action of these enzymes and for identifying inhibitors that can modulate their activity in biochemical pathways. Its utility in research extends to studies on enzyme kinetics, enzyme regulation, and the screening of protease inhibitors, providing insights into the intricate biological processes where proteases play a pivotal role.
N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride (CAS 911-77-3) References
- Isolation of a proteinase with plasminogen-activating activity from Lachesis muta muta (bushmaster) snake venom. | Sanchez, EF., et al. 2000. Arch Biochem Biophys. 378: 131-41. PMID: 10871053
- Perspectives of digestive pest control with proteinase inhibitors that mainly affect the trypsin-like activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). | Pereira, ME., et al. 2005. Braz J Med Biol Res. 38: 1633-41. PMID: 16258632
- Isolation and characterization of a trypsin fraction from the pyloric ceca of chinook salmon (Oncorhynchus tshawytscha). | Kurtovic, I., et al. 2006. Comp Biochem Physiol B Biochem Mol Biol. 143: 432-40. PMID: 16458561
- Characterization of a new platelet aggregating factor from crotoxin Crotalus durissus cascavella venom. | Fonseca, FV., et al. 2006. Protein J. 25: 183-92. PMID: 16729248
- A comparative study of the expression of serine proteinases in quiescent seeds and in developing Canavalia ensiformis plants. | Demartini, DR., et al. 2007. J Exp Bot. 58: 521-32. PMID: 17158110
- BjussuSP-I: a new thrombin-like enzyme isolated from Bothrops jararacussu snake venom. | Sant' Ana, CD., et al. 2008. Comp Biochem Physiol A Mol Integr Physiol. 151: 443-454. PMID: 17466550
- Degradation of raw feather by a novel high molecular weight extracellular protease from newly isolated Bacillus cereus DCUW. | Ghosh, A., et al. 2008. J Ind Microbiol Biotechnol. 35: 825-34. PMID: 18427848
- Characterisation of midgut digestive proteases from the maize stem borer Busseola fusca. | George, D., et al. 2008. Pest Manag Sci. 64: 1151-8. PMID: 18537109
- Digestive proteolytic activity in the Sunn pest, Eurygaster integriceps. | Hosseininaveh, V., et al. 2009. J Insect Sci. 9: 1-11. PMID: 20053125
- Proteolytic activity regarding Sarconesiopsis magellanica (Diptera: Calliphoridae) larval excretions and secretions. | Pinilla, YT., et al. 2013. Acta Trop. 128: 686-91. PMID: 24076089
- Alkaline serine proteases from Helicoverpa armigera: potential candidates for industrial applications. | Akbar, SM. and Sharma, HC. 2017. Arch Insect Biochem Physiol. 94: PMID: 28019702
- Purification and characterization of trypsin from Luphiosilurus alexandri pyloric cecum. | Dos Santos, CWV., et al. 2016. Biochem Biophys Rep. 8: 29-33. PMID: 28955938
- Identification and characterization of a serine protease from Bacillus licheniformis W10: A potential antifungal agent. | Ji, ZL., et al. 2020. Int J Biol Macromol. 145: 594-603. PMID: 31891703
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride, 100 mg | sc-207952 | 100 mg | $68.00 | |||
N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride, 500 mg | sc-207952A | 500 mg | $81.00 | |||
N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride, 5 g | sc-207952B | 5 g | $298.00 |