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MAP-4 Antibody (F-5) - Western Blotting - Image 294498
MAP-4 Antibody (F-5): sc-271361. Western blot analysis of MAP-4 expression in HeLa (A) and Caki-1 (B) whole cell lysates.
MAP-4 Antibody (F-5): sc-271361. Western blot analysis of MAP-4 expression in HeLa (A) and Caki-1 (B) whole cell lysates.

MAP-4 Antibody (F-5): sc-271361

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Datasheets
  • MAP-4 Antibody (F-5) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of MAP-4 of human origin
  • recommended for detection of MAP-4 isoforms 1 and 2 of human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAP-4 Antibody (F-5) for WB applications. These reagents are now offered in bundles with MAP-4 Antibody (F-5) (see ordering information below).
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    Additional MAP Antibodies including MAP-1B, MAP-2, MAP-4, MAP-6D1 and MAP-1S

    MAP-4 Antibody (F-5) is a mouse monoclonal IgG2b antibody that detects MAP-4 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAP-4 is a crucial microtubule-associated protein that plays a significant role in the assembly and stability of microtubules, which are essential components of the cytoskeletal network. MAP-4 influences the spatial organization of microtubules within cells, thereby facilitating various cellular processes, including mitosis and intracellular transport. MAP-4 is primarily expressed in tissues such as the kidney, lung, liver, testis, and spleen, where MAP-4 contributes to the formation of interphase microtubules and the dynamics of mitotic spindle fibers. MAP-4′s structure includes three 18-amino-acid repeats that are homologous to those found in other microtubule-associated proteins, underscoring MAP-4′s evolutionary significance and functional versatility. By promoting microtubule assembly and stability, MAP-4 is vital for maintaining cellular integrity and facilitating proper cell division, making MAP-4 an important target for research in cell biology and related fields.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    MAP-4 Antibody (F-5) References:

    1. Mouse microtubule-associated protein 4 (MAP4) transcript diversity generated by alternative polyadenylation.  |  Code, RJ. and Olmsted, JB. 1992. Gene. 122: 367-70. PMID: 1487151
    2. Site-specific microtubule-associated protein 4 dephosphorylation causes microtubule network densification in pressure overload cardiac hypertrophy.  |  Chinnakkannu, P., et al. 2010. J Biol Chem. 285: 21837-48. PMID: 20436166
    3. Microtubule-associated protein 4 binds to actin filaments and modulates their properties.  |  Matsushima, K., et al. 2012. J Biochem. 151: 99-108. PMID: 21937675
    4. Microtubule-associated protein (MAP) 4 interacts with microtubules in an intrinsically disordered manner.  |  Hashi, Y., et al. 2014. Biosci Biotechnol Biochem. 78: 1864-70. PMID: 25052097
    5. Microtubule-associated protein 4 is an important regulator of cell invasion/migration and a potential therapeutic target in esophageal squamous cell carcinoma.  |  Jiang, YY., et al. 2016. Oncogene. 35: 4846-56. PMID: 26876215
    6. Microtubule-Associated Protein 4 Is a Prognostic Factor and Promotes Tumor Progression in Lung Adenocarcinoma.  |  Xia, X., et al. 2018. Dis Markers. 2018: 8956072. PMID: 29743960
    7. Phosphorylation of Microtubule- Associated Protein 4 Promotes Hypoxic Endothelial Cell Migration and Proliferation.  |  Zhang, J., et al. 2019. Front Pharmacol. 10: 368. PMID: 31040780
    8. Microtubule elongation along actin filaments induced by microtubule-associated protein 4 contributes to the formation of cellular protrusions.  |  Doki, C., et al. 2020. J Biochem. 168: 295-303. PMID: 32289170
    9. MAP4 as a New Candidate in Cardiovascular Disease.  |  Li, L., et al. 2020. Front Physiol. 11: 1044. PMID: 32982783
    10. Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4.  |  Thapa, N., et al. 2020. Nat Cell Biol. 22: 1357-1370. PMID: 33139939

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    MAP-4 Antibody (F-5)

    sc-271361
    200 µg/ml
    		$322.00

    MAP-4 Antibody (F-5): m-IgG Fc BP-HRP Bundle

    sc-537624
    200 µg Ab; 10 µg BP
    		$361.00

    MAP-4 Antibody (F-5): m-IgGκ BP-HRP Bundle

    sc-534942
    200 µg Ab; 40 µg BP
    		$361.00

    What is the recommended fixation method for immunofluorescence staining with MAP-4 (F-5): sc-271361 monoclonal antibody?

    Asked by: TinTin
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Great antibodyThe antibody works very well in 4% PFA fixed cells. I used the dil 1:500 and it worked very well. It also gives a very low background. Great antibody especially for the IF!
    Date published: 2017-12-15
    Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-03-12
    Rated 5 out of 5 by from Strong band in WB in HeLa nuclear extractStrong band in WB in HeLa nuclear extract. -SCBT QC
    Date published: 2015-03-12
    Rated 5 out of 5 by from Positive band obtained in WB in HeLa and CakiPositive band obtained in WB in HeLa and Caki-1 whole cell lysates. -SCBT QC
    Date published: 2015-01-07
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    MAP-4 Antibody (F-5) is rated 5.0 out of 5 by 4.
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