Date published: 2025-9-8

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Anticorps MAP-2 (A-8): sc-74422

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Fiches techniques
  • L'Anticorps MAP-2 (A-8) est un monoclonal de souris IgG2a κ, cité dans 13 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-300 de MAP-2 d'origine human
  • recommandé pour la détection de MAP-2 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • Voir MAP-2 (A-4): sc-74421 pour d'autres anticorps conjugués MAP-2, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • m-IgG2a BP-HRP est le réactif secondaire recommandé pour la détection de l'anticorps MAP-2 (A-8) pour des applications de WB and IHC(P). Ce réactif est maintenant proposé en kit avec l'anticorps MAP-2 (A-8) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubule-associated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance micro-tubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps MAP-2 (A-8)

sc-74422
200 µg/ml
$316.00

MAP-2 (A-8): m-IgG2a BP-HRP Kit

sc-546009
200 µg Ab; 10 µg BP
$354.00

I am using MAP-2 (A-8): sc-74422 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Posée par: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2020-08-11
  • y_2025, m_8, d_28, h_5CST
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  • QUESTIONS, PRODUCT
Rated 5 de 5 de par observed target bandRat brian tissue extracts (50ug),1:800 diluted the primary, and 1:5000 diluted the secondary,The WB result is expected bands at 280kDa.
Date de publication: 2017-07-02
Rated 5 de 5 de par worked good qualityI tried different antibody dilutions and to optimize it, but I always get the same (very strong) bands from 1:500 to 1:800 (Hela cell).
Date de publication: 2017-07-01
Rated 5 de 5 de par Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2015-03-19
Rated 5 de 5 de par Excellent immunoperoxidase neuropil and cytoplasmicExcellent immunoperoxidase neuropil and cytoplasmic staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date de publication: 2015-03-16
Rated 5 de 5 de par Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2015-01-10
Rated 5 de 5 de par Worked well in WB in mouse and rat brainWorked well in WB in mouse and rat brain tissue extracts. -SCBT QC
Date de publication: 2015-01-08
  • y_2025, m_8, d_28, h_5
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Anticorps MAP-2 (A-8) est évalué 5.0 de 5 de 6.
  • y_2025, m_8, d_28, h_5
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  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT