Ly-49F Antibody (5K30): sc-71522
- Ly-49F Antibody (5K30) is a mouse monoclonal IgG1 κ Ly-49F antibody provided at 200 µg/ml
- raised against the B6 allele of Ly-49F of mouse origin
- recommended for detection of Ly-49F of mouse origin by FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Ly-49F Antibody (5K30). This reagent is now offered in a bundle with Ly-49F Antibody (5K30) (see ordering information below).
Ly-49F Antibody (5K30) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ly-49F protein of mouse origin by flow cytometry (FCM). anti-Ly-49F antibody (5K30) is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. Ly-49F protein plays a crucial role in the regulation of natural killer (NK) cell activity, which is essential for the immune response against tumors and virally infected cells. Specifically, Ly-49F is a member of the C-type lectin superfamily of type II membrane glycoproteins, which are known for their ability to recognize class I major histocompatibility complex (MHC) molecules. This recognition helps NK cells distinguish between healthy and abnormal cells, thereby preventing the proliferation of potentially harmful cells. Ly-49F isoform, along with other Ly-49 proteins, is clustered on human chromosome 16 and functions by presenting the C-type lectin domain on the cell membrane, facilitating interactions with MHC class I molecules. Understanding Ly-49F function and interactions is important for developing therapeutic strategies that enhance NK cell responses in cancer immunotherapy and other diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ly-49F Antibody (5K30) References:
- Genes in two major histocompatibility complex class I regions control selection, phenotype, and function of a rat Ly-49 natural killer cell subset. | Naper, C., et al. 1999. Eur J Immunol. 29: 2046-53. PMID: 10382768
- Levels of Ly-49 receptor expression are determined by the frequency of interactions with MHC ligands: evidence against receptor calibration to a 'useful' level. | Manilay, JO., et al. 1999. J Immunol. 163: 2628-33. PMID: 10453002
- Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. | Coles, MC., et al. 2000. Eur J Immunol. 30: 236-44. PMID: 10602046
- Positive and negative roles of the trans-acting T cell factor-1 for the acquisition of distinct Ly-49 MHC class I receptors by NK cells. | Kunz, B. and Held, W. 2001. J Immunol. 166: 6181-7. PMID: 11342639
- Specificity and function of activating Ly-49 receptors. | Kane, KP., et al. 2001. Immunol Rev. 181: 104-14. PMID: 11513132
- Ly-49 receptors and their functions. | Kane, KP., et al. 2004. Crit Rev Immunol. 24: 321-48. PMID: 15663362
- In search of the 'missing self': MHC molecules and NK cell recognition. | Ljunggren, HG. and Kärre, K. 1990. Immunol Today. 11: 237-44. PMID: 2201309
- The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. | Ortaldo, JR., et al. 1998. J Immunol. 160: 1158-65. PMID: 9570529
- Developmentally regulated extinction of Ly-49 receptor expression permits maturation and selection of NK1.1+ T cells. | Robson MacDonald, H., et al. 1998. J Exp Med. 187: 2109-14. PMID: 9625772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ly-49F Antibody (5K30) | sc-71522 | 200 µg/ml | $322.00 | |||
Ly-49F Antibody (5K30): m-IgG Fc BP-HRP Bundle | sc-539372 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ly-49F Antibody (5K30) FITC | sc-71522 FITC | 200 µg/ml | $336.00 | |||
Ly-49F Antibody (5K30) PE | sc-71522 PE | 200 µg/ml | $349.00 |