Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L-type Ca++ CP β4 CRISPR/Cas9 KO Plasmid (m): sc-419412
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-type Ca++ CP β4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L-type Ca++ CP β4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-type Ca++ CP β4 Antibody (H-7): sc-376432
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-type Ca++ CP β4 CRISPR/Cas9 KO Plasmid (m) | sc-419412 | 20 µg | $397.00 |
Overview
Cacnb4 encodes the β4 auxiliary subunit of L-type voltage-gated calcium channels, regulating channel trafficking, membrane stability, and gating kinetics that shape Ca²⁺ influx in excitable tissues. By tuning activity-dependent calcium entry, β4 influences Ca²⁺-dependent signaling cascades that couple membrane depolarization to neurotransmitter release, gene transcription, and synaptic plasticity. In mouse models, altered Cacnb4 function perturbs neuronal excitability and network synchronization, linking this pathway to neurodevelopmental and neuropsychiatric phenotypes as well as seizure susceptibility. The protein’s role in controlling calcium channel composition also makes it relevant for studies of circuit maturation and activity-dependent cellular stress responses.
L-type Ca++ CP β4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cacnb4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cacnb4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cacnb4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L-type Ca++ CP β4 protein expression.
This CRISPR knockout system enables efficient generation of Cacnb4-deficient cell models for investigation of L-type Ca++ CP β4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cacnb4 exon(s) critical for L-type Ca++ CP β4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cacnb4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L-type Ca++ CP β4 CRISPR/Cas9 KO Plasmid (m) and L-type Ca++ CP β4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cacnb4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L-type Ca++ CP β4 HDR Plasmid (m) and L-type Ca++ CP β4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cacnb4 homology arms to support homology-directed repair at defined Cacnb4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.