JFC1 Antibody (F-6): sc-365657

JFC1 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects JFC1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JFC1 Antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JFC1, also known as Slp1 (synaptotagmin-like protein), plays a crucial role in vesicular trafficking, which is essential for processes such as neurotransmitter release and hormone secretion. JFC1 is characterized by two N-terminal Slp homology domains (SHD1 and SHD2), which contain conserved α-helical regions that specifically bind to the GTP-bound form of Rab 27a, a small GTPase involved in regulating vesicle transport. The interaction between JFC1 and Rab 27a is vital for proper localization and function of synaptic vesicles, highlighting JFC1′s importance in maintaining cellular communication and signaling pathways. JFC1 is transcriptionally activated by NFκB and upregulated by TNFα in prostate carcinoma cells, indicating a potential role in cancer biology. JFC1 primarily associates with the plasma membrane, further emphasizing JFC1′s significance in cellular dynamics and signaling.

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JFC1 Antibody (F-6) References:

1. Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins.  |  Fukuda, M. and Mikoshiba, K. 2001. Biochem Biophys Res Commun. 281: 1226-33. PMID: 11243866
2. JFC1, a novel tandem C2 domain-containing protein associated with the leukocyte NADPH oxidase.  |  McAdara Berkowitz, JK., et al. 2001. J Biol Chem. 276: 18855-62. PMID: 11278853
3. Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain.  |  Fukuda, M., et al. 2001. Biochem Biophys Res Commun. 283: 513-9. PMID: 11327731
4. The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain.  |  Kuroda, TS., et al. 2002. J Biol Chem. 277: 9212-8. PMID: 11773082
5. A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport.  |  Strom, M., et al. 2002. J Biol Chem. 277: 25423-30. PMID: 11980908
6. JFC1 is transcriptionally activated by nuclear factor-kappaB and up-regulated by tumour necrosis factor alpha in prostate carcinoma cells.  |  Catz, SD., et al. 2002. Biochem J. 367: 791-9. PMID: 12137562
7. The C2A domain of JFC1 binds to 3'-phosphorylated phosphoinositides and directs plasma membrane association in living cells.  |  Catz, SD., et al. 2002. Proc Natl Acad Sci U S A. 99: 11652-7. PMID: 12189202
8. Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin.  |  Fukuda, M. and Kuroda, TS. 2002. J Biol Chem. 277: 43096-103. PMID: 12221080

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JFC1 Antibody (F-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-JFC1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JFC1 Antibody (F-6) Specifications

Alternate Product Name: JFC1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 471-550

Epitope Target: JFC1

Epitope Species: human

Product Reactivity/Detection: JFC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for JFC1 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred JFC1 antibody is JFC1 Antibody (E-8): sc-365933.