Date published: 2025-10-5

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Anticorps IκB-α (C-21): sc-371

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  • L'anticorps I kappa B- alpha C-21 est un polyclonal IgG de lapin fourni en 100 µg/ml; ou IgG de chèvre; 200 µg/ml, sc-371-G
  • épitope situé au niveau C-terminus de IκB-α d'origine human
  • Anticorps polyclonal arrêté

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

Informations pour la commande

NFKBIA/IkB alpha (H-4): sc-1643 [ Monoclonal recommandé en remplacement de IκB-α (C-21) ]

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps NFKBIA/IkB alpha (H-4)

sc-1643
200 µg/ml
$316.00

NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP Kit

sc-528129
200 µg Ab; 10 µg BP
$354.00

NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP Kit

sc-520432
200 µg Ab, 40 µg BP
$354.00

Anticorps NFKBIA/IkB alpha (H-4) AC

sc-1643 AC
500 µg/ml, 25% agarose
$416.00

Anticorps NFKBIA/IkB alpha (H-4) HRP

sc-1643 HRP
200 µg/ml
$316.00

Anticorps NFKBIA/IkB alpha (H-4) FITC

sc-1643 FITC
200 µg/ml
$330.00

Anticorps NFKBIA/IkB alpha (H-4) PE

sc-1643 PE
200 µg/ml
$343.00

Anticorps NFKBIA/IkB alpha (H-4) Alexa Fluor® 488

sc-1643 AF488
200 µg/ml
$357.00

Anticorps NFKBIA/IkB alpha (H-4) Alexa Fluor® 546

sc-1643 AF546
200 µg/ml
$357.00

Anticorps NFKBIA/IkB alpha (H-4) Alexa Fluor® 594

sc-1643 AF594
200 µg/ml
$357.00

Anticorps NFKBIA/IkB alpha (H-4) Alexa Fluor® 647

sc-1643 AF647
200 µg/ml
$357.00

Anticorps NFKBIA/IkB alpha (H-4) Alexa Fluor® 680

sc-1643 AF680
200 µg/ml
$357.00

Anticorps NFKBIA/IkB alpha (H-4) Alexa Fluor® 790

sc-1643 AF790
200 µg/ml
$357.00

Anticorps NFKBIA/IkB alpha (H-4) TRITC

sc-1643 TRITC
200 µg/ml
$320.00

Hi, I've tried this antibody in WB using 30 ug of rat  liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

Posée par: dany58
Thanks for your feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact our Technical Service by phone or email or by Live Chat if you need support or additional information
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-11-27

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

Posée par: shiba
Thank you for your question about our rabbit polyclonal antibody, sc-371: IkB-alpha (C-21). The expected molecular weight for IkB-alpha is approximately 37 kDa. This protein may also be phosphorylated which is one possible explanation for why you may also be detecting an additional slightly higher molecular weight band in your samples. I would recommend including additional controls in your experiment to confirm if the additional band above 37 kDa is also IkB-alpha, and a result of the binding of the specific primary antibody. One suggestion is to treat your lysates with Lambda Phosphatase: sc-200312 (https://www.scbt.com/scbt/product/lambda-phosphatase?requestFrom=search) to release phosphate groups and observe the difference in detection by Western blot. I would also recommend to perform a secondary antibody control blot (without primary antibody) to confirm that there is not any non-specific binding of your secondary antibody alone. Please contact our Technical Service Department for additional support in troubleshooting this antibody in your research application. https://www.scbt.com/scbt/resources/technical-service
Répondue par: Technical Support
Date de publication: 2018-12-18
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Rated 5 de 5 de par Good for western blotFor western blot, use at 1:1000 in 5% milk TBST, overnight incubation with rocking at 4 degrees C. Example of published result: PMID 29950426
Date de publication: 2020-01-09
Rated 5 de 5 de par IkB antibody in rat samplesIt is working very well in the rat samples without unspecific bands
Date de publication: 2019-08-02
Rated 5 de 5 de par quality of anti-IkBa antibodyThis antibody's quality is very good, and works very well in our hand.
Date de publication: 2019-01-15
Rated 4 de 5 de par Nice primary antibodyThe primary antibody is available for Western blotting in mice sample.
Date de publication: 2017-06-13
Rated 5 de 5 de par Works really wellI have used this antibody for nearly five years and it worked really well with bone-marrow derived dendritic cell extracts of BALB/c and C57BL/6 mice. There were no non-specific bands and the band intensity for the specific bands was also very good.
Date de publication: 2017-06-06
Rated 4 de 5 de par Excellent experience.I bought this antobody for my current research project involved in the IkB-alpha protein detection by western blot assay for the mice kidney samples, this antibody works very well!
Date de publication: 2017-02-12
Rated 5 de 5 de par Very clean immunoblots and high sensitivityThis is a great antibody for immunoblot analisis of endogenous IkBalpha. Very clean western blots and it can be diluted even 1:5000.
Date de publication: 2016-12-21
Rated 5 de 5 de par Great result in WBGreat result in WB using human and rat lungs tissue.
Date de publication: 2016-11-04
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Anticorps IκB-α (C-21) est évalué 4.8 de 5 de 30.
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