Anticorps Id2 (E-7) recommandé pour la détection de Id2 d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps Id2 (E-7): sc-398104

Anticorps Id2 (E-7) est évalué 4.7 de 5 de 7.
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Fiches techniques

    • Anti-L'anticorps Id2 E-7 est un monoclonal IgG2a L'Anticorps Id2, cité dans 3 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 105-134 au niveau C-terminus de Id2 d'origine mouse
    • Anti-L'Anticorps Id2 (E-7) est recommandé pour la détection de Id2 d'origine mouse, rat et human par WB, IP, IF et ELISA
    • Anti-L'Anticorps Id2 (E-7) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-398104 X, 200 µg/0.1 ml)
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Id2 (E-7): sc-398104.
    • m-IgG Fc BP-HRP et m-IgG2a BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps Id2 (E-7) pour des applications de WB. Ces réactifs sont maintenant proposés en kit avec l'anticorps Id2 (E-7) (voir Informations pour la commande ci-dessous).
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can it used for pig?

Posée par: huarenwu
Thank you for your question. We have not yet tested this antibody, Id2 (E-7): sc-398104, for use in pig samples so we do not recommend it for use with this species at this time. The antibody is raised against amino acids 105-134 at the C-terminus of Id2 of mouse origin. Based on sequence analysis, this sequence shares 90% sequence identity with Id2 of pig origin (protein accession number Q2VIU1). This is a high degree of identity, so there is a good chance that the antibody may also cross-react with pig.
Répondue par: Technical Support
Date de publication: 2017-09-13

What application is the blocking peptide sc-398104 P appropriate for?

Posée par: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 4 de 5 de par Good antibody for Id2 detection by Western blot We tested this antibody on differentiating N1E-115 mouse cell line. It gives a clear signal at about 20 kDa, in line with the expected molecular weight of the protein (17,5 kDa). Background is very minimal. Antibody dilution: 1:250 in PBST with 5% nonfat dry milk.
Date de publication: 2021-02-17
Rated 4 de 5 de par some background, but strong signal in WB diluted 1:100 in TBST, 5% BSA the AB detects endogenous and recombinant fusion protein in WB in NSCLC cell lines with some, yet weaker background
Date de publication: 2017-11-18
Rated 5 de 5 de par Antibody effect is very good we are using renal tubular epithelial cells, with IF detection of Id2 expression, dilution ratio is 1: 80, the results are very good,Fluorescence is shown in the nucleus,antibody effect is very good.
Date de publication: 2017-10-12
Rated 5 de 5 de par Great features Antibody effect is very good, we are using HT22 cells, with western blot detection of Id2 expression, dilution ratio is 1: 500, the results are very good.
Date de publication: 2017-07-17
Rated 5 de 5 de par For therapy The Id2 antibody we used was mouse monoclonal IgG2a provided at 200 μg / ml, as suggested by WB, IP, IF, and ELISA as shown in the figure, and we performed a perfect procedure for targeted therapy
Date de publication: 2017-02-09
Rated 5 de 5 de par Versatile and Clean Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date de publication: 2017-01-27
Rated 5 de 5 de par Produced positive Western blot data of Id2 Produced positive Western blot data of Id2 expression in non-transfected and mouse Id2 transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2014-09-07
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