HGFA Double Nickase Plasmid (h): sc-404422-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HGFA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HGFA Double Nickase Plasmid (h) and HGFA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HGFAC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HGFA Antibody (B-6): sc-515126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HGFA Double Nickase Plasmid (h) | sc-404422-NIC | 20 µg | $410.00 | |||
HGFA Double Nickase Plasmid (h2) | sc-404422-NIC-2 | 20 µg | $410.00 |
HGFAC encodes hepatocyte growth factor activator (HGFA), a secreted serine protease that converts single-chain pro-HGF into active HGF, thereby potentiating MET receptor signaling. This proteolytic step links extracellular protease networks to downstream pathways controlling epithelial–mesenchymal interactions, cell motility, proliferation, and tissue remodeling, with crosstalk to coagulation- and fibrinolysis-associated cascades. Dysregulated HGF/HGFA–MET axis activity has been implicated in abnormal stromal–epithelial signaling, invasive growth programs, and altered regenerative responses, making HGFAC a useful node for mechanistic studies of pericellular proteolysis and growth factor activation in human cells.
HGFA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HGFAC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HGFAC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HGFAC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HGFAC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.