Grancalcin Antibody (G-3): sc-365808
- Grancalcin Antibody (G-3) is a mouse monoclonal IgG1 κ Grancalcin antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 21-55 near the N-terminus of Grancalcin of human origin
- Grancalcin Antibody (G-3) is recommended for detection of Grancalcin of human origin by WB, IP, IF and ELISA
- Anti-Grancalcin Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Grancalcin Antibody (G-3) for WB applications. These reagents are now offered in bundles with Grancalcin Antibody (G-3) (see ordering information below).
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Grancalcin Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Grancalcin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Grancalcin (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Grancalcin is a calcium-binding protein predominantly found in human neutrophils and plays a crucial role in immune response regulation. Grancalcin belongs to the penta EF-hand (PEF) subfamily of EF-hand proteins, which includes calpain, sorcin, peflin, and ALG-2. Grancalcin′s structure is characterized by forming a homodimer through the association of unpaired EF5 hands, which is essential for function. Upon calcium binding, Grancalcin undergoes significant conformational changes that expose hydrophobic amino acid residues, allowing interaction with hydrophobic surfaces and potentially influencing cellular processes. Notably, Grancalcin interacts with L-plastin, a protein known for actin bundling activity, suggesting involvement in regulating neutrophil adhesion and migration. This interaction highlights Grancalcin′s importance in the immune system, particularly in inflammation and pathogen response, as Grancalcin is primarily associated with cells originating from the bone marrow, especially neutrophils and monocytes, while only trace amounts are found in lymphocytes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Grancalcin Antibody (G-3) References:
- Crystal structure of human grancalcin, a member of the penta-EF-hand protein family. | Jia, J., et al. 2000. J Mol Biol. 300: 1271-81. PMID: 10903868
- Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. | Lollike, K., et al. 2001. J Biol Chem. 276: 17762-9. PMID: 11279160
- Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils. | Teahan, CG., et al. 1992. Biochem J. 286 (Pt 2): 549-54. PMID: 1530588
- Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes. | Boyhan, A., et al. 1992. J Biol Chem. 267: 2928-33. PMID: 1737748
- An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes. | Lollike, K., et al. 1995. J Immunol Methods. 185: 1-8. PMID: 7665891
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Grancalcin Antibody (G-3) | sc-365808 | 200 µg/ml | $322.00 | |||
Grancalcin Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-529476 | 200 µg Ab; 10 µg BP | $361.00 | |||
Grancalcin Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-522377 | 200 µg Ab, 40 µg BP | $361.00 | |||
Grancalcin Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-543532 | 200 µg Ab; 20 µg BP | $361.00 | |||
Grancalcin Antibody (G-3) AC | sc-365808 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Grancalcin Antibody (G-3) HRP | sc-365808 HRP | 200 µg/ml | $322.00 | |||
Grancalcin Antibody (G-3) FITC | sc-365808 FITC | 200 µg/ml | $336.00 | |||
Grancalcin Antibody (G-3) PE | sc-365808 PE | 200 µg/ml | $349.00 | |||
Grancalcin Antibody (G-3) Alexa Fluor® 488 | sc-365808 AF488 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (G-3) Alexa Fluor® 546 | sc-365808 AF546 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (G-3) Alexa Fluor® 594 | sc-365808 AF594 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (G-3) Alexa Fluor® 647 | sc-365808 AF647 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (G-3) Alexa Fluor® 680 | sc-365808 AF680 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (G-3) Alexa Fluor® 790 | sc-365808 AF790 | 200 µg/ml | $364.00 | |||
Grancalcin (G-3) Neutralizing Peptide | sc-365808 P | 100 µg/0.5 ml | $69.00 |