GP-39 Antibody (E-11): sc-376910

GP-39 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects GP-39 of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GP-39 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted primarily by articular chondrocytes, synoviocytes, and macrophages, playing a crucial role in the inflammatory response. Elevated levels of GP-39 in serum and synovial fluid are associated with inflammatory diseases, particularly rheumatoid arthritis, where GP-39 levels correlate with the extent of joint damage. GP-39 is predominantly expressed in articular chondrocytes and synovial cells, with additional expression in the liver, while GP-39 remains undetectable in muscle tissues, lung, pancreas, mononuclear cells, and fibroblasts. GP-39′s structure, characterized by a unique combination of a chitin-binding domain and a fibronectin type III-like domain, is essential for GP-39′s interaction with other proteins and role in modulating the immune response. GP-39 is considered a candidate autoantigen in rheumatoid arthritis, highlighting GP-39′s importance in the ability of cells to adapt to environmental changes and maintain homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GP-39 Antibody (E-11) References:

1. Human cartilage glycoprotein 39 (HC gp-39) mRNA expression in adult and fetal chondrocytes, osteoblasts and osteocytes by in-situ hybridization.  |  Connor, JR., et al. 2000. Osteoarthritis Cartilage. 8: 87-95. PMID: 10772238
2. GP-83 and GP-39, two glycoproteins secreted by human epididymis are conjugated to spermatozoa during maturation.  |  Liu, HW., et al. 2000. Mol Hum Reprod. 6: 422-8. PMID: 10775645
3. YKL-40 (cartilage gp-39) induces proliferative events in cultured chondrocytes and synoviocytes and increases glycosaminoglycan synthesis in chondrocytes.  |  De Ceuninck, F., et al. 2001. Biochem Biophys Res Commun. 285: 926-31. PMID: 11467840
4. Analysis of chondrex (YKL-40, HC gp-39) in the cerebrospinal fluid of patients with spine disease.  |  Tsuji, T., et al. 2002. Spine (Phila Pa 1976). 27: 732-5. PMID: 11923666
5. The chitinase 3-like protein human cartilage glycoprotein 39 (HC-gp39) stimulates proliferation of human connective-tissue cells and activates both extracellular signal-regulated kinase- and protein kinase B-mediated signalling pathways.  |  Recklies, AD., et al. 2002. Biochem J. 365: 119-26. PMID: 12071845
6. Localization of MHC class II/human cartilage glycoprotein-39 complexes in synovia of rheumatoid arthritis patients using complex-specific monoclonal antibodies.  |  Steenbakkers, PG., et al. 2003. J Immunol. 170: 5719-27. PMID: 12759455
7. HC gp-39 gene is upregulated in glioblastomas.  |  Shostak, K., et al. 2003. Cancer Lett. 198: 203-10. PMID: 12957359
8. Detection of major histocompatibility complex/human cartilage gp-39 complexes in rheumatoid arthritis synovitis as a specific and independent histologic marker.  |  Baeten, D., et al. 2004. Arthritis Rheum. 50: 444-51. PMID: 14872486
9. Functions of CD40 and Its Ligand, gp39 (CD40L).  |  Laman, JD., et al. 2017. Crit Rev Immunol. 37: 371-420. PMID: 29773027
10. The role of CD40L (gp39)/CD40 in T/B cell interaction and primary immunodeficiency.  |  Ochs, HD., et al. 1994. Semin Immunol. 6: 337-41. PMID: 7532462
11. The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.  |  Aruffo, A., et al. 1993. Cell. 72: 291-300. PMID: 7678782
12. Human cartilage gp-39, a major secretory product of articular chondrocytes and synovial cells, is a mammalian member of a chitinase protein family.  |  Hakala, BE., et al. 1993. J Biol Chem. 268: 25803-10. PMID: 8245017

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GP-39 Antibody (E-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-GP-39 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GP-39 Antibody (E-11) Specifications

Alternate Product Name: GP-39

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 136-178

Epitope Target: GP-39

Epitope Species: mouse

Product Reactivity/Detection: GP-39

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for GP-39 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin