Gβ Antibody (C-8): sc-166064
- Gβ Antibody (C-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 302-340 at the C-terminus of Gβ of mouse origin
- recommended for detection of Gβ1, Gβ2, Gβ3 and Gβ4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Gβ Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Gβ Antibody (C-8) (see ordering information below).
Gβ Antibody (C-8) is a mouse monoclonal IgG1 antibody that detects Gβ in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Gβ, a crucial component of heterotrimeric G proteins, plays a significant role in transmitting signals from cell surface receptors to intracellular effectors, thereby influencing numerous physiological processes. Gβ subunits are essential regulators of G protein α subunits and certain signal transduction receptors, which are vital for maintaining cellular communication and response to external stimuli. Notably, Gβ5, one of the five members of the β-subunit family, exhibits unique characteristics, being only 50% homologous to the other β subunits, Gβ1-4, which share at least 83% homology. This distinctiveness is important as Gβ5 is predominantly expressed in the brain, pancreas, kidney, and heart, suggesting specialized functions in these tissues. anti-Gβ antibody (C-8) is an invaluable tool for researchers aiming to explore the intricate signaling pathways mediated by G proteins, facilitating a deeper understanding of their roles in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gβ Antibody (C-8) References:
- A conserved Gbeta binding (GBB) sequence motif in Ste20p/PAK family protein kinases. | Leberer, E., et al. 2000. Biol Chem. 381: 427-31. PMID: 10937873
- Chronic morphine acts via a protein kinase Cgamma-G(beta)-adenylyl cyclase complex to augment phosphorylation of G(beta) and G(betagamma) stimulatory adenylyl cyclase signaling. | Chakrabarti, S., et al. 2005. Brain Res Mol Brain Res. 138: 94-103. PMID: 15908039
- Characterization of glycerophosphodiesterase 4-interacting molecules Gαq/11 and Gβ, which mediate cellular lysophospholipase D activity. | Aoyama, C., et al. 2019. Biochem J. 476: 3721-3736. PMID: 31794025
- Multi-omics analysis of glucose-mediated signaling by a moonlighting Gβ protein Asc1/RACK1. | Li, S., et al. 2021. PLoS Genet. 17: e1009640. PMID: 34214075
- Interplay between ARABIDOPSIS Gβ and WRKY transcription factors differentiates environmental stress responses. | Boonyaves, K., et al. 2022. Plant Physiol. 190: 813-827. PMID: 35748759
- Structural Basis of the Interaction of the G Proteins, Gαi1, Gβ1γ2 and Gαi1β1γ2, with Membrane Microdomains and Their Relationship to Cell Localization and Activity. | Álvarez, R. and Escribá, PV. 2023. Biomedicines. 11: PMID: 36831093
- Signal profiles and spatial regulation of β-arrestin recruitment through Gβ5 and GRK3 at the μ-opioid receptor. | Carino, CMC., et al. 2025. Eur J Pharmacol. 987: 177151. PMID: 39579957
- G beta gamma interactions with PH domains and Ras-MAPK signaling pathways. | Inglese, J., et al. 1995. Trends Biochem Sci. 20: 151-6. PMID: 7770915
- Protein intake affects levels of G-protein subunits G alpha i2, G alpha i3, and G beta in rat glomerular membranes. | Yanagisawa, H., et al. 1993. Kidney Int. 43: 1068-73. PMID: 8510384
- A putative alpha-helical G beta gamma-coupling domain in the second intracellular loop of the 5-HT1A receptor. | Albert, PR., et al. 1998. Ann N Y Acad Sci. 861: 146-61. PMID: 9928252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gβ Antibody (C-8) | sc-166064 | 200 µg/ml | $322.00 | |||
Gβ Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-540004 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gβ Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-541839 | 200 µg Ab; 20 µg BP | $361.00 | |||
Gβ (C-8) Neutralizing Peptide | sc-166064 P | 100 µg/0.5 ml | $69.00 |