Date published: 2025-9-9

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Anticorps FOXE3 (D-6): sc-377465

4.5(2)
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Fiches techniques
  • L'Anticorps FOXE3 (D-6) est un monoclonal de souris IgG1 κ, cité dans 3 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 232-288 situés au niveau C-terminus de FOXE3 d'origine mouse
  • recommandé pour la détection de FOXE3 d'origine mouse, rat et human par WB, IP, IF et ELISA
  • Réactifs TransCruz pour une application en ChIP (sc-377465 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs de détection secondaire préférés pour FOXE3 Antibody (D-6) for WB applications. Ces réactifs sont désormais proposés en lots avec FOXE3 Antibody (D-6)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The human forkhead-box (FOX) gene family consists of at least 43 members, including FOXE3, a 288-amino acid protein. FOXE3 is a winged-helix transcription factor that plays a crucial role during the initial stages of lens development and closure of the lens vesicle. FOXE3 may also act as a factor that promotes survival and proliferation while preventing differentiation in the lens epithelium. As the posterior cells of the lens fiber begin to differentiate, expression of FOXE3 is limited to the undifferentiated cells coating the anterior surface of the lens. Congenital primary aphakia (CPA) is a rare developmental disorder caused by a null mutation in the FOXE3 gene that is identified by the absence of a lens. The development of CPA is normally stimulated during the fourth or fifth week of human embryogenesis.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps FOXE3 (D-6)

sc-377465
200 µg/ml
$316.00

FOXE3 (D-6): m-IgG Fc BP-HRP Kit

sc-540723
200 µg Ab; 10 µg BP
$354.00

FOXE3 (D-6): m-IgG1 BP-HRP Kit

sc-542325
200 µg Ab; 20 µg BP
$354.00

Anticorps FOXE3 (D-6) X

sc-377465 X
200 µg/0.1 ml
$316.00

Why does the blot show two different bands or could you precise what does each band correspond to ?

Posée par: Cangee
Thank you for your question. The western blot image shows cell lysates that were transfected with a truncated peptide corresponding to the antibody's binding site. Endogenous FOXE3 will produce a signal at 33 kDa in western blots.
Répondue par: Tech Support Europe
Date de publication: 2022-03-31

Why does the blot show two bands, and neither of which is at 33 kDa (which is the predicted protein size for human FOXE3 protein)?

Posée par: SPadula
Thank you for your question. The Western blot data may not be representative of the endogenous molecular weight for FOXE3. This is detected in a human FOXE3 transfected 293T lysate. The observed molecular weight of FOXE3 may be higher than the predicted molecular weight as a result of post-translational modifications of the protein.
Répondue par: Technical Support
Date de publication: 2022-03-31

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-377465: FOXE3 (D-6) mouse monoclonal antibody will work?

Posée par: jenniferc
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Répondue par: Technical Support
Date de publication: 2017-03-06
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Rated 4 de 5 de par Hard to set, but works fineWe bought this product for a western blot on mouse lens epithelium. It took some time to find the right parameters to run the experiment, but when we found them, the antibody worked fine for our samples, and allowed quantification of FOXE3. The only problem remains the aspecific band at 50kDa, it would be interesting to understand what it corresponds to (obviously not FOXE3).
Date de publication: 2023-08-21
Rated 5 de 5 de par Works well in WB in mouse FOXP1 transfectedWorks well in WB in mouse FOXP1 transfected 293T whole cell lysate. -SCBT QC
Date de publication: 2014-09-30
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Anticorps FOXE3 (D-6) est évalué 4.5 de 5 de 2.
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