Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERRα Double Nickase Plasmid (h): sc-401772-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERRα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ERRα Double Nickase Plasmid (h) and ERRα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ESRRA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ERRα Antibody (2ERR2): sc-65718
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERRα Double Nickase Plasmid (h) | sc-401772-NIC | 20 µg | $410.00 | |||
ERRα Double Nickase Plasmid (h2) | sc-401772-NIC-2 | 20 µg | $410.00 |
ESRRA encodes estrogen-related receptor alpha (ERRα), an orphan nuclear receptor that functions as a transcriptional regulator of mitochondrial biogenesis and oxidative metabolism. ERRα cooperates with coactivators such as PPARGC1A/PGC-1α to control gene programs involved in fatty acid oxidation, oxidative phosphorylation, and cellular energy homeostasis. Through integration with AMPK, mTOR, and hypoxia-responsive signaling, ERRα influences metabolic adaptation, proliferation, and differentiation in multiple tissues. Dysregulated ESRRA activity has been associated with altered metabolic phenotypes and has been investigated in contexts including cancer metabolism, cardiometabolic dysfunction, and muscle and bone biology.
ERRα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ESRRA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ESRRA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ESRRA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ESRRA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.