Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ephrin-B1 CRISPR/Cas9 KO Plasmid (h): sc-400873
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ephrin-B1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ephrin-B1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ephrin-B1 Antibody (C-6): sc-515264
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ephrin-B1 CRISPR/Cas9 KO Plasmid (h) | sc-400873 | 20 µg | $397.00 |
Overview
EFNB1 encodes ephrin-B1, a membrane-tethered ligand for Eph receptor tyrosine kinases that mediates contact-dependent signaling controlling cell positioning, boundary formation, and tissue morphogenesis. Ephrin-B1 participates in bidirectional Eph/ephrin signaling, integrating with Rho family GTPase regulation, actin cytoskeletal remodeling, and adherens junction dynamics to influence migration and adhesion. Through these processes it contributes to angiogenesis, axon guidance, and epithelial organization, and its dysregulation has been linked to developmental abnormalities and altered cell–cell communication in cancer-related pathways. EFNB1 is also relevant to studies of X-linked gene dosage and mosaicism, providing a model for understanding how local signaling interfaces shape tissue patterning.
ephrin-B1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EFNB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EFNB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EFNB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ephrin-B1 protein expression.
This CRISPR knockout system enables efficient generation of EFNB1-deficient cell models for investigation of ephrin-B1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EFNB1 exon(s) critical for ephrin-B1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EFNB1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ephrin-B1 CRISPR/Cas9 KO Plasmid (h) and ephrin-B1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EFNB1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ephrin-B1 HDR Plasmid (h) and ephrin-B1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EFNB1 homology arms to support homology-directed repair at defined EFNB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.