Anticorps EphB3 (7E5) recommandé pour la détection de EphB3 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps EphB3 (7E5): sc-100299

Anticorps EphB3 (7E5) est évalué 4.6 de 5 de 5.
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Fiches techniques

    • L'Anticorps EphB3 (7E5) est un monoclonal de souris IgG1 (chaîne légère kappa) fourni en 100 µg/ml
    • dirigé contre la EphB3 recombinante d'origine human
    • recommandé pour la détection de EphB3 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.

Can sc-100299: EphB3 (7E5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: cjMara
Thank you for your question. Yes, sc-100299: EphB3 (7E5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 4 de 5 de par Produced good immunoperoxidase membrane staining Produced good immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date de publication: 2015-04-16
Rated 4 de 5 de par Positive detection of endogenous EphB3 protein Positive detection of endogenous EphB3 protein by WB using HeLa whole cell lysate. -SCBT QC
Date de publication: 2015-03-04
Rated 5 de 5 de par Publishable data obtained by WB and IHC using Publishable data obtained by WB and IHC using mouse bone marrow. -SCBT Publication Review
Date de publication: 2015-02-16
Rated 5 de 5 de par Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human EphB3 protein via Western Blot analysis. -SCBT QC
Date de publication: 2015-02-03
Rated 5 de 5 de par An increase in signal was observed via Western An increase in signal was observed via Western Blot when comparing mouse EphB3 transfected 293T cells with those that have not been transfected to over express the protein of interest. -SCBT QC
Date de publication: 2015-01-19
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