Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4AII CRISPR/Cas9 KO Plasmid (h): sc-402758
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4AII CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF4AII genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4AII Antibody (H-5): sc-137148
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4AII CRISPR/Cas9 KO Plasmid (h) | sc-402758 | 20 µg | $397.00 |
Overview
EIF4A2 encodes the human ATP-dependent RNA helicase eIF4AII, a core component of the eIF4F translation initiation machinery that remodels 5′ UTR secondary structure to facilitate cap-dependent ribosome scanning. By controlling mRNA recruitment and start codon recognition, eIF4AII influences global and transcript-selective protein synthesis programs tied to cell growth, stress adaptation, and differentiation. Its activity intersects with RNA metabolism pathways and translational control nodes that integrate upstream signaling inputs affecting proliferation and survival. Dysregulated translation initiation, including altered eIF4A family function, is frequently implicated in oncogenic proteome remodeling and other disorders linked to aberrant mRNA regulation.
eIF4AII CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EIF4A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EIF4A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EIF4A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF4AII protein expression.
This CRISPR knockout system enables efficient generation of EIF4A2-deficient cell models for investigation of eIF4AII signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EIF4A2 exon(s) critical for eIF4AII function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EIF4A2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF4AII CRISPR/Cas9 KO Plasmid (h) and eIF4AII CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EIF4A2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF4AII HDR Plasmid (h) and eIF4AII HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EIF4A2 homology arms to support homology-directed repair at defined EIF4A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.