Date published: 2025-9-8

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Anticorps eIF3ζ (A-3): sc-271516

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Fiches techniques
  • L'Anticorps eIF3ζ (A-3) est un monoclonal de souris IgG1 κ, cité dans 1 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-300 situés au niveau N-terminus de eIF3ζ d'origine human
  • recommandé pour la détection de eIF3ζ d'origine mouse, rat et human par WB, IP, IF et ELISA
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRP et m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps eIF3ζ (A-3) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps eIF3ζ (A-3)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that lead to 80S ribosomal assembly and, ultimately, translation. The eukaryotic initiation factor-3 (eIF3) scaffolding structure is the largest of the eIF complexes and includes eIF3α, eIF3β, eIF3γ, eIF3δ, eIF3ε, eIF3ζ, eIF3η and eIF3θ, all of which function to control the assembly of the 40S ribosomal subunit. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex. Overexpression of eIF3 proteins is common in several cancers, suggesting a role for eIF3 proteins in tumorigenesis.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps eIF3ζ (A-3)

sc-271516
200 µg/ml
$316.00

eIF3ζ (A-3): m-IgG Fc BP-HRP Kit

sc-537650
200 µg Ab; 10 µg BP
$354.00

eIF3ζ (A-3): m-IgGκ BP-HRP Kit

sc-534984
200 µg Ab; 40 µg BP
$354.00

eIF3ζ (A-3): m-IgG1 BP-HRP Kit

sc-545283
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with eIF3ζ (A-3): sc-271516 monoclonal antibody?

Posée par: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-02-27
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Rated 5 de 5 de par Good specificity in WB in PCGood specificity in WB in PC-12 whole cell lysate. -SCBT QC
Date de publication: 2015-05-12
Rated 5 de 5 de par Worked well in WB in 293T whole cell lysateWorked well in WB in 293T whole cell lysate. -SCBT QC
Date de publication: 2015-03-30
Rated 5 de 5 de par Good cytoplasmic staining in HeLa cells in IFGood cytoplasmic staining in HeLa cells in IF. -SCBT QC
Date de publication: 2015-03-24
Rated 5 de 5 de par Strong band in WB in mouse MEF cellsStrong band in WB in mouse MEF cells. -SCBT Publication Review
Date de publication: 2014-11-04
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Anticorps eIF3ζ (A-3) est évalué 5.0 de 5 de 4.
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