EF-2 Antibody (F-9): sc-166409
- EF-2 Antibody (F-9) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 829-856 near the C-terminus of EF-2 of human origin
- recommended for detection of EF-2 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EF-2 Antibody (F-9) for WB and IHC(P) applications. These reagents are now offered in bundles with EF-2 Antibody (F-9) (see ordering information below).
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EF-2 Antibody (F-9) is a mouse monoclonal IgG1 antibody that detects EF-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EF-2, or elongation factor 2, is a crucial GTPase that plays a vital role in the elongation phase of protein synthesis on the ribosome, facilitating the translocation of tRNAs and mRNAs. This process is essential for ensuring that the ribosome can accurately decode mRNA sequences into proteins, which are fundamental for cellular function and growth. The importance of EF-2 lies in its ability to support the movement of the ribosome along the mRNA strand, allowing for the sequential addition of amino acids to the growing polypeptide chain. Additionally, EF-2′s interaction with the ribosome is tightly regulated by GTP binding and hydrolysis, which is critical for maintaining the fidelity and efficiency of protein synthesis. Anti-EF-2 antibody (F-9) enables researchers to gain insights into the dynamics of protein translation and the regulatory mechanisms that govern this essential biological process.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EF-2 Antibody (F-9) References:
- Elongation factor-2 phosphorylation in dendrites and the regulation of dendritic mRNA translation in neurons. | Heise, C., et al. 2014. Front Cell Neurosci. 8: 35. PMID: 24574971
- Eukaryotic elongation factor 2 kinase confers tolerance to stress conditions in cancer cells. | Zhu, H., et al. 2015. Cell Stress Chaperones. 20: 217-20. PMID: 25248493
- Eukaryotic Elongation Factor 2 Kinase (eEF2K) in Cancer. | Wang, X., et al. 2017. Cancers (Basel). 9: PMID: 29186827
- Structural basis of elongation factor 2 switching. | Fenwick, MK. and Ealick, SE. 2020. Curr Res Struct Biol. 2: 25-34. PMID: 34235467
- Insights Into the Pathologic Roles and Regulation of Eukaryotic Elongation Factor-2 Kinase. | Ballard, DJ., et al. 2021. Front Mol Biosci. 8: 727863. PMID: 34532346
- Roles of eukaryotic elongation factor 2 kinase (eEF2K) in neuronal plasticity, cognition, and Alzheimer disease. | Ma, T. 2023. J Neurochem. 166: 47-57. PMID: 34796967
- Control of CD4+ T cells to restrain inflammatory diseases via eukaryotic elongation factor 2 kinase. | Peng, HY., et al. 2023. Signal Transduct Target Ther. 8: 415. PMID: 37875468
- The aryl hydrocarbon receptor controls mesenchymal stromal cell-mediated immunomodulation via ubiquitination of eukaryotic elongation factor-2 kinase. | Lkhagva-Yondon, E., et al. 2023. Cell Death Dis. 14: 812. PMID: 38071243
- Targeting eukaryotic elongation factor 2 kinase (eEF2K) with small-molecule inhibitors for cancer therapy. | Wang, H., et al. 2024. Drug Discov Today. 29: 104155. PMID: 39214495
- Elongation factor 2 in cancer: a promising therapeutic target in protein translation. | Jia, X., et al. 2024. Cell Mol Biol Lett. 29: 156. PMID: 39707196
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-2 Antibody (F-9) | sc-166409 | 200 µg/ml | $322.00 | |||
EF-2 Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-537485 | 200 µg Ab; 10 µg BP | $361.00 | |||
EF-2 Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-534764 | 200 µg Ab; 40 µg BP | $361.00 | |||
EF-2 Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-545201 | 200 µg Ab; 20 µg BP | $361.00 | |||
EF-2 (F-9) Neutralizing Peptide | sc-166409 P | 100 µg/0.5 ml | $69.00 |