E4F1 Antibody (D-12): sc-514718
- E4F1 Antibody (D-12) is a mouse monoclonal IgG2a κ E4F1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-240 mapping at the N-terminus of E4F1 of human origin
- E4F1 Antibody (D-12) is recommended for detection of E4F1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-E4F1 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for E4F1 Antibody (D-12) for WB applications. These reagents are now offered in bundles with E4F1 Antibody (D-12) (see ordering information below).
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E4F1 Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects E4F1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E4F1 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E4F1, also known as E4F transcription factor 1, is a 784 amino acid protein found in both nucleus and cytoplasm, where E4F1 plays a crucial role in regulating gene expression. E4F1 contains nine C2H2-type zinc fingers, essential for DNA-binding capabilities, allowing E4F1 to function as a transcriptional repressor. This regulation maintains cellular homeostasis and influences cell survival and growth through cell cycle control. E4F1 also acts as a ubiquitin ligase, facilitating ubiquitination and subsequent degradation of target proteins, participating in the p53 tumor suppressor pathway. E4F1 undergoes post-translational modifications such as phosphorylation and sumoylation, and proteolytic cleavage, resulting in a short peptide with specific DNA-binding properties, highlighting E4F1′s multifaceted role in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E4F1 Antibody (D-12) References:
- p53 is involved in the p120E4F-mediated growth arrest. | Sandy, P., et al. 2000. Oncogene. 19: 188-99. PMID: 10644996
- pRB binds to and modulates the transrepressing activity of the E1A-regulated transcription factor p120E4F. | Fajas, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 7738-43. PMID: 10869426
- Identification of the E1A-regulated transcription factor p120 E4F as an interacting partner of the RASSF1A candidate tumor suppressor gene. | Fenton, SL., et al. 2004. Cancer Res. 64: 102-7. PMID: 14729613
- E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis. | Nakamura, Y., et al. 2004. Am J Pathol. 165: 2019-31. PMID: 15579445
- The LIM-only protein FHL2 is a negative regulator of E4F1. | Paul, C., et al. 2006. Oncogene. 25: 5475-84. PMID: 16652157
- E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation. | Le Cam, L., et al. 2006. Cell. 127: 775-88. PMID: 17110336
- The adenovirus E1A-regulated transcription factor E4F is generated from the human homolog of nuclear factor phiAP3. | Fernandes, ER. and Rooney, RJ. 1997. Mol Cell Biol. 17: 1890-903. PMID: 9121437
- Chromosomal location and tissue expression of the gene encoding the adenovirus E1A-regulated transcription factor E4F in humans and mice. | Rooney, RJ., et al. 1998. Mamm Genome. 9: 320-3. PMID: 9530632
- Assignment of the E1A-regulated transcription factor E4F gene (E4F1) to human chromosome band 16p13.3 by in situ hybridization and somatic cell hybrids. | Saccone, S., et al. 1998. Cytogenet Cell Genet. 82: 99-100. PMID: 9763670
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E4F1 Antibody (D-12) | sc-514718 | 200 µg/ml | $322.00 | |||
E4F1 Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-531204 | 200 µg Ab; 10 µg BP | $361.00 | |||
E4F1 Antibody (D-12): m-IgGκ BP-HRP Bundle | sc-524825 | 200 µg Ab, 40 µg BP | $361.00 | |||
E4F1 Antibody (D-12): m-IgG2a BP-HRP Bundle | sc-547725 | 200 µg Ab; 10 µg BP | $361.00 | |||
E4F1 Antibody (D-12) AC | sc-514718 AC | 500 µg/ml, 25% agarose | $424.00 | |||
E4F1 Antibody (D-12) HRP | sc-514718 HRP | 200 µg/ml | $322.00 | |||
E4F1 Antibody (D-12) FITC | sc-514718 FITC | 200 µg/ml | $336.00 | |||
E4F1 Antibody (D-12) PE | sc-514718 PE | 200 µg/ml | $349.00 | |||
E4F1 Antibody (D-12) Alexa Fluor® 488 | sc-514718 AF488 | 200 µg/ml | $364.00 | |||
E4F1 Antibody (D-12) Alexa Fluor® 546 | sc-514718 AF546 | 200 µg/ml | $364.00 | |||
E4F1 Antibody (D-12) Alexa Fluor® 594 | sc-514718 AF594 | 200 µg/ml | $364.00 | |||
E4F1 Antibody (D-12) Alexa Fluor® 647 | sc-514718 AF647 | 200 µg/ml | $364.00 | |||
E4F1 Antibody (D-12) Alexa Fluor® 680 | sc-514718 AF680 | 200 µg/ml | $364.00 | |||
E4F1 Antibody (D-12) Alexa Fluor® 790 | sc-514718 AF790 | 200 µg/ml | $364.00 |