Dvl-2/Dishevelled 2/DVL2 CRISPR/Cas9 KO Plasmid (m): sc-420081

Overview

Mouse Dvl2 encodes Dishevelled 2 (DVL2), a cytoplasmic scaffold protein that transduces signals from Frizzled receptors to downstream Wnt pathways. DVL2 integrates canonical Wnt/β-catenin signaling with non-canonical planar cell polarity and Wnt/Ca²⁺ cascades by coordinating receptor-proximal complexes and regulating cytoskeletal dynamics, polarity, and trafficking. Through these roles, DVL2 contributes to embryonic patterning, stem/progenitor cell behavior, and tissue homeostasis, and its dysregulation is frequently examined in contexts of aberrant Wnt signaling linked to developmental defects and oncogenic signaling programs. Dvl2 is also studied for effects on cell migration and morphogenesis, where pathway balance between β-catenin–dependent transcription and PCP outputs is critical.

Dvl-2/Dishevelled 2/DVL2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dvl2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dvl2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dvl2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dvl-2/Dishevelled 2/DVL2 protein expression.

This CRISPR knockout system enables efficient generation of Dvl2-deficient cell models for investigation of Dvl-2/Dishevelled 2/DVL2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Dvl2 exon(s) critical for Dvl-2/Dishevelled 2/DVL2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Dvl2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Dvl-2/Dishevelled 2/DVL2 CRISPR/Cas9 KO Plasmid (m) and Dvl-2/Dishevelled 2/DVL2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dvl2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Dvl-2/Dishevelled 2/DVL2 HDR Plasmid (m) and Dvl-2/Dishevelled 2/DVL2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dvl2 homology arms to support homology-directed repair at defined Dvl2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.