Dvl-1/Dishevelled 1/DVL1 CRISPR/Cas9 KO Plasmid (h): sc-400785

Overview

DVL1 encodes Dishevelled 1 (Dvl-1), a cytoplasmic phosphoprotein that functions as a core scaffold in Wnt signal transduction. Dvl-1 integrates receptor-proximal inputs from Frizzled and co-receptors to propagate both β-catenin–dependent (canonical) and planar cell polarity/Wnt–Ca²⁺ (non-canonical) signaling, influencing cell polarity, migration, and transcriptional programs. Through its DIX, PDZ, and DEP domains, Dvl-1 coordinates multiprotein complexes that regulate β-catenin stabilization, small GTPase signaling, and cytoskeletal remodeling. Dysregulation of Wnt/Dishevelled signaling is frequently linked to altered developmental patterning and oncogenic pathway activation, making DVL1 a relevant node for mechanistic studies of pathway rewiring in disease-associated contexts.

Dvl-1/Dishevelled 1/DVL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DVL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DVL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DVL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dvl-1/Dishevelled 1/DVL1 protein expression.

This CRISPR knockout system enables efficient generation of DVL1-deficient cell models for investigation of Dvl-1/Dishevelled 1/DVL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting DVL1 exon(s) critical for Dvl-1/Dishevelled 1/DVL1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple DVL1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Dvl-1/Dishevelled 1/DVL1 CRISPR/Cas9 KO Plasmid (h) and Dvl-1/Dishevelled 1/DVL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DVL1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Dvl-1/Dishevelled 1/DVL1 HDR Plasmid (h) and Dvl-1/Dishevelled 1/DVL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DVL1 homology arms to support homology-directed repair at defined DVL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.