DDI1 CRISPR/Cas9 KO Plasmid (m): sc-428086
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDI1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDI1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDI1 CRISPR/Cas9 KO Plasmid (m) | sc-428086 | 20 µg | $397.00 |
Overview
Mouse DDI1 (DNA damage-inducible 1 homolog) encodes a conserved protein implicated in ubiquitin-dependent proteostasis through its ubiquitin-like and ubiquitin-associated domains and a retroviral protease-like region. DDI1 has been linked to regulation of protein turnover, stress adaptation, and crosstalk with DNA damage responses, consistent with roles in maintaining protein quality control and cellular homeostasis. Perturbation of DDI1-associated pathways can influence accumulation of ubiquitinated proteins and stress signaling outputs that are relevant to models of neurodegeneration, cancer biology, and inflammatory responses. In mouse systems, DDI1 provides a tractable node for studying how ubiquitin-mediated pathways intersect with genome stability and proteotoxic stress.
DDI1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ddi1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ddi1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ddi1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDI1 protein expression.
This CRISPR knockout system enables efficient generation of Ddi1-deficient cell models for investigation of DDI1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ddi1 exon(s) critical for DDI1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ddi1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDI1 CRISPR/Cas9 KO Plasmid (m) and DDI1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ddi1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDI1 HDR Plasmid (m) and DDI1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ddi1 homology arms to support homology-directed repair at defined Ddi1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.