Date published: 2025-9-7

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Anticorps CstF-50 (H-8): sc-393317

5.0(2)
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Fiches techniques
  • L'Anticorps CstF-50 (H-8) est un monoclonal de souris mouse IgG2b κ (chaîne légère kappa) fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-300 situés au niveau N-terminus de CstF-50 d'origine human
  • recommandé pour la détection de CstF-50 d'origine mouse, rat et human par WB, IP, IF et ELISA; également réactif avec d'autres espèces, dont et equine, canine, bovine and porcine
  • Le m-IgG Fc BP-HRP et le m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps CstF-50 (H-8) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps CstF-50 (H-8)(voir les informations relatives à la commande ci-dessous).

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. The shortest CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44-amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3′-end cleavage factors to form a stable complex on the pre-mRNA.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps CstF-50 (H-8)

    sc-393317
    200 µg/ml
    $316.00

    CstF-50 (H-8): m-IgG Fc BP-HRP Kit

    sc-538187
    200 µg Ab; 10 µg BP
    $354.00

    CstF-50 (H-8): m-IgGκ BP-HRP Kit

    sc-535751
    200 µg Ab; 40 µg BP
    $354.00

    How much sample do you recommend loading on the gel for Western blot detection using CstF-50 (H-8): sc-393317?

    Posée par: Randy McDonald
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Répondue par: Technical Support
    Date de publication: 2017-03-01
    • y_2025, m_8, d_27, h_5CST
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    Rated 5 de 5 de par Produced positive Western Blot data of CstFProduced positive Western Blot data of CstF-50 expression in A-431, Jurkat, K-562 and SK-BR-3 nuclear extracts and HeLa whole cell lysate . -SCBT QC
    Date de publication: 2015-02-24
    Rated 5 de 5 de par Great Western Blot data of CstFGreat Western Blot data of CstF-50 expression in non-transfected and human CstF-50 transfected 293T whole cell lysates. -SCBT QC
    Date de publication: 2014-11-26
    • y_2025, m_8, d_27, h_5
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    Anticorps CstF-50 (H-8) est évalué 5.0 de 5 de 2.
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