Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. The shortest CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44-amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3′-end cleavage factors to form a stable complex on the pre-mRNA.
Informations pour la commande
Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
Anticorps CstF-50 (G-3) | sc-393962 | 200 µg/ml | $316.00 | |||
CstF-50 (G-3): m-IgG Fc BP-HRP Kit | sc-538346 | 200 µg Ab; 10 µg BP | $354.00 | |||
CstF-50 (G-3): m-IgGκ BP-HRP Kit | sc-535939 | 200 µg Ab; 40 µg BP | $354.00 | |||
CstF-50 (G-3): m-IgG1 BP-HRP Kit | sc-545654 | 200 µg Ab; 20 µg BP | $354.00 | |||
CstF-50 (G-3) peptide neutralisant | sc-393962 P | 100 µg/0.5 ml | $68.00 |