CRMP-3 CRISPR/Cas9 KO Plasmid (h): sc-404504
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRMP-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRMP-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRMP-3 CRISPR/Cas9 KO Plasmid (h) | sc-404504 | 20 µg | $397.00 |
Overview
DPYSL4 encodes collapsin response mediator protein 3 (CRMP-3), a cytosolic phosphoprotein in the CRMP family that links extracellular guidance cues to microtubule dynamics and cytoskeletal remodeling. CRMP-3 has been implicated in neurite outgrowth, axon guidance, and regulation of cellular morphology through signaling pathways downstream of semaphorins and related kinases that modulate CRMP phosphorylation states. Beyond neural contexts, DPYSL4 expression has been associated with stress-adaptive programs and metabolic rewiring, connecting it to processes such as mitochondrial function, motility, and cell survival. Dysregulated DPYSL4/CRMP-3 signaling has been reported in studies of neurological disorders and cancer-associated phenotypes, supporting its utility as a mechanistic target for pathway interrogation.
CRMP-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DPYSL4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DPYSL4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DPYSL4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRMP-3 protein expression.
This CRISPR knockout system enables efficient generation of DPYSL4-deficient cell models for investigation of CRMP-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DPYSL4 exon(s) critical for CRMP-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DPYSL4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRMP-3 CRISPR/Cas9 KO Plasmid (h) and CRMP-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DPYSL4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRMP-3 HDR Plasmid (h) and CRMP-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DPYSL4 homology arms to support homology-directed repair at defined DPYSL4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.