Date published: 2025-9-11

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Anticorps COX5b (W10): sc-100523

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Fiches techniques
  • L'Anticorps COX5b (W10) est un monoclonal de souris IgG1 κ, cité dans 1 publications, fourni en 100 µg/ml
  • dirigé contre la COX5b recombinante d'origine human
  • recommandé pour la détection de COX5b d'origine human par WB, IP, IF et ELISA
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire COX5b (W10). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps COX5b (W10)

sc-100523
100 µg/ml
$333.00

I am using COX5b (W10): sc-100523 for immunofluorescence, what dilution of this antibody should I use?

Posée par: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
  • y_2025, m_8, d_29, h_8CST
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Rated 4 de 5 de par Good immunofluorescence cytoplasmic stainingGood immunofluorescence cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date de publication: 2015-03-20
Rated 3 de 5 de par Band detected in WB using Hep G2 whole cell lysateBand detected in WB using Hep G2 whole cell lysate. -SCBT QC
Date de publication: 2015-01-17
Rated 4 de 5 de par Publishable data in WB using human embryonicPublishable data in WB using human embryonic cell lines. -SCBT Publication Review
Date de publication: 2015-01-10
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Anticorps COX5b (W10) est évalué 3.7 de 5 de 3.
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