Anticorps Coronin 1A (14.1) recommandé pour la détection de Coronin 1A d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Coronin 1A (14.1): sc-100925

Anticorps Coronin 1A (14.1) est évalué 4.0 de 5 de 7.
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Fiches techniques

    • Anti-L'anticorps Coronin 1A 14.1 est un monoclonal IgG2a κ L'Anticorps Coronin 1A, cité dans 7 publications, fourni en 100 µg/ml
    • raised against a C-terminal region of Coronin 1A of human origin
    • recommandé pour la détection de Coronin 1A d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Coronin 1A (14.1): sc-100925.

Is Coronin 1A (14.1) monoclonal antibody suitable for IHC/IF?

Posée par: Ariamino
Thank you for your question. Yes, this mouse monoclonal antibody, Coronin 1A (14.1): sc-100925, is recommended for IHC(P) and IF. Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, as well as immunofluorescence on fresh or frozen tissues or cells.
Répondue par: Technical Support
Date de publication: 2017-10-25

I was using coronin1A antibody (sc-366201). I was planning to order it again but the problem is that the current list of coronin 1a antibodies is not having this one on this website. What should I do?

Posée par: Hriday S Pandey
Thank you for your question. Our rabbit polyclonal antibody, sc-366201: Coronin 1A (H-300) is now discontinued. As an alternative, we would recommend using our new mouse monoclonal antibody, Coronin 1A (14.1): sc-100925. https://www.scbt.com/scbt/product/coronin-1a-antibody-14-1
Répondue par: Technical Support
Date de publication: 2017-09-28

I was using coronin1A antibody (sc-366201). I was planning to order it again but the problem is that the current list of coronin 1a antibodies on the website is not having this one. What should I do?

Posée par: Hriday
Thank you for your question. That rabbit polyclonal antibody, sc-366201, is now discontinued. We do offer a suggested replacement mouse monoclonal antibody, Coronin 1A (14.1): sc-100925. https://www.scbt.com/scbt/product/coronin-1a-antibody-14-1
Répondue par: Technical Support
Date de publication: 2017-09-27

Can sc-100925: Coronin 1A (14.1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: TinTin
Thank you for your question. Yes, sc-100925: Coronin 1A (14.1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 2 de 5 de par Very weak signal Used a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. Detected a single band but the signal was very weak.
Date de publication: 2018-01-30
Rated 5 de 5 de par WB for mouse brain was worked well. WB for mouse brain was worked well. Signal intensity was strong.
Date de publication: 2017-12-09
Rated 5 de 5 de par Great IHC and WB antibody Observed specific stain and desired band with Coronin 1A Antibody (14.1)
Date de publication: 2017-01-23
Rated 4 de 5 de par WB publishable data with NCI WB publishable data with NCI-H441 cell line and female BALB/c mice - SCBT Publication Review
Date de publication: 2015-04-29
Rated 4 de 5 de par Good specificity in WB in K Good specificity in WB in K-562 whole cell lysate. -SCBT QC
Date de publication: 2015-04-13
Rated 4 de 5 de par Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2015-01-15
Rated 4 de 5 de par Produced good immunoperoxidase nuclear and Produced good immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human lung tissue. -SCBT QC
Date de publication: 2014-02-28
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