Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Contactin 1 CRISPR/Cas9 KO Plasmid (h): sc-403437
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Contactin 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Contactin 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Contactin 1 Antibody (41): sc-136133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Contactin 1 CRISPR/Cas9 KO Plasmid (h) | sc-403437 | 20 µg | $397.00 |
Overview
CNTN1 encodes Contactin 1, a glycosylphosphatidylinositol (GPI)-anchored immunoglobulin superfamily cell adhesion molecule enriched in the nervous system. Contactin 1 participates in axon guidance, neurite outgrowth, and the organization of paranodal junctions by coordinating interactions between neurons and glia that support saltatory conduction. Through its role in cell–cell adhesion and membrane microdomain organization, CNTN1 influences cytoskeletal dynamics and neuronal connectivity during development and synaptic remodeling. Altered CNTN1 expression or function has been linked to neurodevelopmental and neurodegenerative phenotypes and is also reported in studies of tumor cell adhesion, migration, and invasive behavior.
Contactin 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CNTN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CNTN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CNTN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Contactin 1 protein expression.
This CRISPR knockout system enables efficient generation of CNTN1-deficient cell models for investigation of Contactin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CNTN1 exon(s) critical for Contactin 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CNTN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Contactin 1 CRISPR/Cas9 KO Plasmid (h) and Contactin 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CNTN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Contactin 1 HDR Plasmid (h) and Contactin 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CNTN1 homology arms to support homology-directed repair at defined CNTN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.