Clusterin-α Antibody (A-11): sc-166831
- Clusterin-α Antibody (A-11) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 432-449 at the C-terminus of Clusterin-α of human origin
- Anti-Clusterin-alpha Antibody (A-11) is recommended for detection of Clusterin-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Clusterin-alpha Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Clusterin-α Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Clusterin-α Antibody (A-11) (see ordering information below).
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Clusterin-α Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody engineered to recognize Clusterin-α of human origin by targeting an epitope mapped between amino acids 432-449 at the C-terminus. Clusterin-α monoclonal antibody (A-11) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), demonstrating robust reactivity with Clusterin-α from mouse, rat, and human species. Clusterin-α, also known as apolipoprotein J (ApoJ) or complement lysis inhibitor (CLI), is a multifunctional secretory heterodimeric glycoprotein composed of α and β subunits linked by disulfide bonds, formed through the post-translational cleavage of a 449 amino acid precursor at the internal bond between Arg227 and Ser228. The structural integrity of Clusterin-α is crucial for its diverse roles in various physiological processes, including immune regulation, cell adhesion, lipid transportation, tissue remodeling, membrane recycling, and cell-cell interactions. Functionally, Clusterin-α acts as a key modulator of the complement system by inhibiting the formation of the membrane attack complex, thereby protecting cells from complement-mediated lysis and maintaining immune homeostasis. Additionally, Clusterin-α interacts with apoptotic proteins such as Bax, regulating apoptosis and promoting cell survival under stress conditions. In the nervous system, Clusterin-α binds to amyloid-beta peptides, influencing their aggregation and reducing neuritic toxicity, which is significant in the pathogenesis of Alzheimer′s disease. Moreover, Clusterin-α interacts with various extracellular matrix proteins, facilitating cell adhesion and migration, which are essential for tissue remodeling and cancer metastasis. Overexpression of Clusterin-α has been observed in advanced stages of several cancers, including mammary tumors, where Clusterin-α supports tumor progression and metastasis by enhancing cell survival and resistance to apoptosis. Anti-Clusterin-α antibody (A-11) enables detailed studies of Clusterin-α′s interactions with these critical proteins, providing valuable insights into its role in immune regulation, apoptosis, neuroprotection, and cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clusterin-α Antibody (A-11) | sc-166831 | 200 µg/ml | $322.00 | |||
Clusterin-α Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-529063 | 200 µg Ab; 10 µg BP | $361.00 | |||
Clusterin-α Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-521762 | 200 µg Ab, 40 µg BP | $361.00 | |||
Clusterin-α Antibody (A-11): m-IgG2a BP-HRP Bundle | sc-547253 | 200 µg Ab; 10 µg BP | $361.00 | |||
Clusterin-α Antibody (A-11) AC | sc-166831 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Clusterin-α Antibody (A-11) HRP | sc-166831 HRP | 200 µg/ml | $322.00 | |||
Clusterin-α Antibody (A-11) FITC | sc-166831 FITC | 200 µg/ml | $336.00 | |||
Clusterin-α Antibody (A-11) PE | sc-166831 PE | 200 µg/ml | $349.00 | |||
Clusterin-α Antibody (A-11) Alexa Fluor® 488 | sc-166831 AF488 | 200 µg/ml | $364.00 | |||
Clusterin-α Antibody (A-11) Alexa Fluor® 546 | sc-166831 AF546 | 200 µg/ml | $364.00 | |||
Clusterin-α Antibody (A-11) Alexa Fluor® 594 | sc-166831 AF594 | 200 µg/ml | $364.00 | |||
Clusterin-α Antibody (A-11) Alexa Fluor® 647 | sc-166831 AF647 | 200 µg/ml | $364.00 | |||
Clusterin-α Antibody (A-11) Alexa Fluor® 680 | sc-166831 AF680 | 200 µg/ml | $364.00 | |||
Clusterin-α Antibody (A-11) Alexa Fluor® 790 | sc-166831 AF790 | 200 µg/ml | $364.00 | |||
Clusterin-α (A-11) Neutralizing Peptide | sc-166831 P | 100 µg/0.5 ml | $69.00 |