Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cerberus CRISPR/Cas9 KO Plasmid (h): sc-417362
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cerberus CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cerberus genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cerberus Antibody (C-1): sc-515324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cerberus CRISPR/Cas9 KO Plasmid (h) | sc-417362 | 20 µg | $397.00 |
Overview
CER1 encodes cerberus, a secreted antagonist of key morphogen pathways that binds and inhibits members of the BMP, NODAL/TGF-β, and WNT families to modulate extracellular signaling gradients. Through these interactions, cerberus contributes to embryonic patterning decisions, including mesendoderm specification and anterior neural development, by constraining SMAD- and β-catenin–dependent transcriptional programs. Dysregulated CER1 expression has been reported in contexts involving aberrant differentiation, epithelial–mesenchymal transition, and altered growth factor signaling, making it relevant to studies of developmental disorders and oncogenic pathway rewiring. As a secreted regulator, CER1 is also useful for interrogating paracrine control of cell fate in stem cell differentiation and organoid models.
cerberus CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CER1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CER1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CER1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cerberus protein expression.
This CRISPR knockout system enables efficient generation of CER1-deficient cell models for investigation of cerberus signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CER1 exon(s) critical for cerberus function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CER1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cerberus CRISPR/Cas9 KO Plasmid (h) and cerberus CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CER1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cerberus HDR Plasmid (h) and cerberus HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CER1 homology arms to support homology-directed repair at defined CER1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.