Cdc4 Antibody (3D1): sc-293423

Cdc4 Antibody (3D1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cdc4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Cdc4 antibody (3D1) is available as the non-conjugated format. Cdc4 (3D1) monoclonal antibody recognizes a protein that plays a crucial role in regulating the cell cycle, particularly in the transition from G1 to S phase, which is essential for proper cell division and proliferation. Cdc4 functions as a component of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex, facilitating the ubiquitination and subsequent degradation of specific target proteins, including the S-phase cyclin/Cdk inhibitor Sic1. This degradation enables the progression of the cell cycle by allowing activation of cyclin-dependent kinases necessary for the transition into S phase. Cdc4 also interacts with other proteins in the ubiquitin-protein ligase complex, such as Cdc53, which helps target phosphorylated G1 cyclins for degradation, ensuring tight cell cycle regulation. Cdc4′s role in mediating these processes highlights its importance in maintaining cellular homeostasis and preventing uncontrolled cell growth, which can lead to cancer.

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Cdc4 Antibody (3D1) References:

1. Control of genomic instability and epithelial tumor development by the p53-Fbxw7/Cdc4 pathway.  |  Perez-Losada, J., et al. 2005. Cancer Res. 65: 6488-92. PMID: 16061623
2. CDC4 mutations occur in a subset of colorectal cancers but are not predicted to cause loss of function and are not associated with chromosomal instability.  |  Kemp, Z., et al. 2005. Cancer Res. 65: 11361-6. PMID: 16357143
3. Loss of CDC4/FBXW7 in gastric carcinoma.  |  Milne, AN., et al. 2010. Cell Oncol. 32: 347-59. PMID: 20448329
4. An allosteric conduit facilitates dynamic multisite substrate recognition by the SCFCdc4 ubiquitin ligase.  |  Csizmok, V., et al. 2017. Nat Commun. 8: 13943. PMID: 28045046
5. Structural comparison of the yeast cell division cycle gene CDC4 and a related pseudogene.  |  Yochem, J. and Byers, B. 1987. J Mol Biol. 195: 233-45. PMID: 3309335
6. Starting to cycle: G1 controls regulating cell division in budding yeast.  |  Sherlock, G. and Rosamond, J. 1993. J Gen Microbiol. 139: 2531-41. PMID: 8277239
7. Control of the yeast cell cycle by the Cdc28 protein kinase.  |  Nasmyth, K. 1993. Curr Opin Cell Biol. 5: 166-79. PMID: 8507488
8. Cdc53 targets phosphorylated G1 cyclins for degradation by the ubiquitin proteolytic pathway.  |  Willems, AR., et al. 1996. Cell. 86: 453-63. PMID: 8756727
9. The transcription factor Swi5 regulates expression of the cyclin kinase inhibitor p40SIC1.  |  Knapp, D., et al. 1996. Mol Cell Biol. 16: 5701-7. PMID: 8816483
10. Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities.  |  Levine, K., et al. 1996. Mol Cell Biol. 16: 6794-803. PMID: 8943334
11. Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast.  |  Deshaies, RJ. 1997. Curr Opin Genet Dev. 7: 7-16. PMID: 9024629
12. SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities.  |  Verma, R., et al. 1997. Mol Biol Cell. 8: 1427-37. PMID: 9285816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc4 Antibody (3D1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc4 Antibody (3D1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc4 Antibody (3D1) Specifications

Alternate Product Name: Cdc4

Product Clone Name: 3D1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 599-707

Epitope Target: Cdc4

Epitope Species: human

Product Reactivity/Detection: Cdc4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Cdc4 Antibody (3D1): N/A

The preferred Cdc4 antibody is Cdc4 Antibody (A-2): sc-518091.