CD45RB Antibody (BRA11): sc-66202
- CD45RB Antibody (BRA11) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against CALLA positive acute lymphoid lukemia cell line REH of human origin
- CD45RB Antibody (BRA11) is recommended for detection of CD45RB of human origin by WB, IP, IF and IHC(P)
- Anti-CD45RB Antibody (BRA11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD45RB Antibody (BRA11) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45RB Antibody (BRA11) (see ordering information below).
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CD45RB Antibody (BRA11) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the CD45RB isoform of CD45 protein, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45RB is a crucial transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in humans, playing an essential role in regulating immune cell signaling and activation. Structurally, CD45RB comprises an extracellular domain generated through alternative splicing of exons 4, 5, and 6, a single transmembrane region, and a conserved intracellular domain that possesses phosphatase activity. This structural configuration enables CD45RB to interact with different cell surface molecules, thereby modulating key signal transduction pathways involved in T and B cell receptor signaling. Located on the cell membrane, CD45RB functions as a phosphotyrosine phosphatase, dephosphorylating specific tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70. These interactions drive lymphocyte development, activation, and immune responses. CD45RB undergoes significant post-translational modifications, including extensive glycosylation, which are crucial for stability, proper localization on the cell membrane, and interactions with other signaling proteins. Anti-CD45RB antibody (BRA11) is raised against human peripheral blood lymphocytes (PBL) maintained in T-cell growth factor and is effective in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD45RB monoclonal antibody (BRA11) facilitates comprehensive analysis of immune cell populations and their interactions with key signaling proteins, providing valuable insights into immune system dynamics and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD45RB Antibody (BRA11) References:
- Ligation of CD45 and the isoforms CD45RA and CD45RB accelerates the rate of constitutive apoptosis in human eosinophils. | Blaylock, MG., et al. 1999. J Allergy Clin Immunol. 104: 1244-50. PMID: 10589008
- Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. | Bazil, V., et al. 1989. Immunogenetics. 29: 202-5. PMID: 2466778
- Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. | Johnson, P., et al. 1989. J Exp Med. 169: 1179-84. PMID: 2466938
- IL-18Rα-deficient CD4(+) T cells induce intestinal inflammation in the CD45RB(hi) transfer model of colitis despite impaired innate responsiveness. | Holmkvist, P., et al. 2016. Eur J Immunol. 46: 1371-82. PMID: 27062602
- Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. | Streuli, M., et al. 1987. J Exp Med. 166: 1548-66. PMID: 2824653
- Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. | Poppema, S., et al. 1987. Am J Pathol. 127: 418-29. PMID: 3296769
- The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections. | West, KP., et al. 1986. J Pathol. 150: 89-101. PMID: 3540244
- New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections. | Hall, PA., et al. 1987. J Clin Pathol. 40: 151-6. PMID: 3546393
- Regulation of immune function by protein tyrosine phosphatases. | Okumura, M. and Thomas, ML. 1995. Curr Opin Immunol. 7: 312-9. PMID: 7546394
- Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. | Trowbridge, IS. 1978. J Exp Med. 148: 313-23. PMID: 78961
- CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. | Trowbridge, IS. and Thomas, ML. 1994. Annu Rev Immunol. 12: 85-116. PMID: 8011300
- Pathogenic and protective roles of CD45RB(low) CD4+ cells correlate with cytokine profiles in the spontaneously autoimmune diabetic mouse. | Shimada, A., et al. 1996. Diabetes. 45: 71-8. PMID: 8522063
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45RB Antibody (BRA11) | sc-66202 | 200 µg/ml | $322.00 | |||
CD45RB Antibody (BRA11): m-IgG Fc BP-HRP Bundle | sc-528647 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD45RB Antibody (BRA11): m-IgGκ BP-HRP Bundle | sc-521084 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD45RB Antibody (BRA11): m-IgG1 BP-HRP Bundle | sc-543051 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD45RB Antibody (BRA11) AC | sc-66202 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD45RB Antibody (BRA11) HRP | sc-66202 HRP | 200 µg/ml | $322.00 | |||
CD45RB Antibody (BRA11) FITC | sc-66202 FITC | 200 µg/ml | $336.00 | |||
CD45RB Antibody (BRA11) PE | sc-66202 PE | 200 µg/ml | $349.00 | |||
CD45RB Antibody (BRA11) Alexa Fluor® 488 | sc-66202 AF488 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (BRA11) Alexa Fluor® 546 | sc-66202 AF546 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (BRA11) Alexa Fluor® 594 | sc-66202 AF594 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (BRA11) Alexa Fluor® 647 | sc-66202 AF647 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (BRA11) Alexa Fluor® 680 | sc-66202 AF680 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (BRA11) Alexa Fluor® 790 | sc-66202 AF790 | 200 µg/ml | $364.00 |