CD45 Antibody (3H1379): sc-70700

CD45 Antibody (3H1379) is a rat monoclonal IgG2b antibody engineered to target CD45 protein, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a vital transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in mice, playing a crucial role in regulating immune cell signaling and activation. Structurally, CD45 consists of an extracellular domain with multiple isoforms generated through alternative splicing of exons 4, 5, and 6, a single transmembrane region, and a conserved intracellular domain that possesses phosphatase activity. This structural organization is essential for CD45′s function, as the variability in the extracellular domain allows for differential interactions with other cell surface molecules, thereby modulating signal transduction pathways critical for T and B cell receptor signaling. Located on the cell membrane, CD45 dephosphorylates key tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70, which are essential for initiating and sustaining signaling cascades that drive lymphocyte development, activation, and immune responses. Additionally, CD45 undergoes significant post-translational modifications, including extensive glycosylation, which are crucial for stability, proper localization on the cell membrane, and interactions with other signaling proteins, thereby enhancing regulatory functions. CD45 monoclonal antibody (3H1379) is raised against spleen cells of mouse origin and is effective in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-CD45 antibody (3H1379) facilitates comprehensive analysis of immune cell populations and their interactions with key signaling proteins, providing valuable insights into immune system dynamics and the mechanisms underlying immune-related diseases. CD45 antibody (3H1379) can detect multiple isoforms of CD45, resulting from alternative splicing, allowing researchers to investigate the diversity and function of different immune cell subsets, making anti-CD45 antibody (3H1379) an indispensable tool for both research and diagnostic purposes in immunology and hematology.

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.