Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
ACCÈS RAPIDE AUX LIENS
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD28 Antibody (3H1179) is a high quality monoclonal CD28 antibody (also designated CD28 antibody) suitable for the detection of the CD28 protein of mouse, rat and human origin. CD28 Antibody (3H1179) is available as the non-conjugated anti-CD28 antibody. T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2), expressed on antigen presenting cells, bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4). The dominant co-stimulatory receptor CD28 is expressed on resting T-cells, while CTLA-4 is expressed as a co-inhibitory receptor on activated T-cells, and binds CD80 and CD86 with higher affinity then CD28. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Does this product stimulate (along with CD3 Ab) rat T cells?
Posée par: David17
Thank you for your question. We are not aware of any references supporting the use of this antibody, CD28 (3H1179): sc-70612, for the purpose of stimulation or other functional activity.
We are aware of one publication citing use of our CD3 Antibody (21-L5): sc-1175 for activation of T-cells. Please see PMID: 8761298 for the product citation.
Répondue par: Technical Support
Date de publication: 2017-06-14
For Western Blot, is it recommended to use denatured or non-denatured conditions with CD28 (3H1179): sc-70612 monoclonal antibody?
Posée par: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
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