Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CCS CRISPR/Cas9 KO Plasmid (h): sc-402130
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CCS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CCS Antibody (H-7): sc-55561
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCS CRISPR/Cas9 KO Plasmid (h) | sc-402130 | 20 µg | $397.00 |
Overview
CCS (copper chaperone for superoxide dismutase) is a cytosolic metallochaperone that delivers copper to SOD1 and promotes its maturation through direct interaction and disulfide bond formation. By enabling proper SOD1 activity, CCS supports cellular antioxidant defense and limits oxidative damage arising from mitochondrial respiration and other redox-active processes. CCS function is linked to copper homeostasis and redox signaling, with downstream effects on stress-responsive pathways that influence protein stability and cellular viability. Dysregulation of the CCS–SOD1 axis has been studied in contexts of oxidative stress biology and neurodegeneration, where altered metal handling and reactive oxygen species contribute to disease-associated phenotypes.
CCS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CCS protein expression.
This CRISPR knockout system enables efficient generation of CCS-deficient cell models for investigation of CCS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCS exon(s) critical for CCS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CCS CRISPR/Cas9 KO Plasmid (h) and CCS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CCS HDR Plasmid (h) and CCS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCS homology arms to support homology-directed repair at defined CCS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.