Anticorps CBP/KAT3A/CREBBP (C-1): sc-7300
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- Anti-L'anticorps CBP/KAT3A/CREBBP C-1 est un monoclonal IgG1 κ L'Anticorps CBP/KAT3A/CREBBP, cité dans 130 publications, fourni en 200 µg/ml
- specific for an epitope mapping between amino acids 2423-2442 at the C-terminus of CBP of human origin (identical to corresponding mouse sequence)
- L'Anticorps CBP/KAT3A/CREBBP (C-1) est recommandé pour la détection de CBP p265 d'origine mouse, rat, human et par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont et canine, bovine, porcine and avian
- Anti-L'Anticorps CBP/KAT3A/CREBBP (C-1) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-7300 X, 200 µg/0.1 ml)
- Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CBP/KAT3A/CREBBP (C-1): sc-7300.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CBP/KAT3A/CREBBP Antibody (C-1). This work is in progress.
ACCÈS RAPIDE AUX LIENS
VOIR ÉGALEMENT...
- Les Produits Chimiques ChemCruz™ CREB Binding Protein (CBP) Inhibiteurs pour l'analyse fonctionnelle des réponses cellulaires de CREB Binding Protein (CBP)
Do you have a directly conjugated isotype control for this antibody. I bought sc-7300 AF594 and need a proper control to show that the positive signal is not a non-specific binding
Thank you for your question. The appropriate isotype control for AF594-conjugated sc-7300 is normal mouse IgG1 Alexa Fluor® 594: sc-516620.
Date de publication: 2020-09-07
I recently purchased this antibody. However, I need to know if this antibody cross-reacts with the closely related p300 protein (KAT3B aka EP300) in human cells. A rapid and accurate response would be greatly appreciated. Thanks, Isaac
Thank you for your question. This mouse monoclonal antibody, CBP (C-1): sc-7300, is specific for CBP (CREB-binding protein). We have not specifically tested this clone for potential cross-reactivity with KAT3B aka EP300, however this antibody has been validated for specificity using our human siRNA for knockdown against the human gene target, CBP siRNA (h): sc-29244.
Date de publication: 2019-06-24
antibody Cat#sc-7300: how do I get in possession of the 10ug sample as advertised on your website? Thanks
Thank you for your question. For a limited time, we are offering a free 10 µg sample of our mouse monoclonal antibody, CBP Antibody (C-1): sc-7300. Please contact our Technical Service Department and we would be happy to arrange a complimentary shipment for you. You may call us toll free at (800) 457-3801 option 2, or email [email protected], or use our live chat!
Date de publication: 2017-01-25
Is there any data showing this antibody is specific to CBP?
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to CBP.
Date de publication: 2020-08-10
Rated 5 de
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oobianom par
Looks visible and great
This antibody works well with transfer buffer that included 0.1%SDS and only 10% methanol. Use for incubation at 1:500
Date de publication: 2018-12-12
Rated 5 de
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Newt112 par
Works in ChIP analysis
In ChIP analysis, tThis antibody could detect forskolin-dependent binding of CBP to c-Fos promoter in HEK293FT cells.
Date de publication: 2018-10-29
Rated 1 de
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julien par
not working for me in IFA
I was unfortunately not successful in getting a signal with 96 well plate probed with recombinant CBP tagged protein O/N at 4C using a fluorescent secondary antibody for 1hr. Antibodies were concentrated as high as 1:50 in PBS-BSA and incubated for 1-2hrs at RT. I used an anti-CBP from a different vendor that gave me a specific signal. This was also tried on fixed cell lines expressing CBP on the surface with negative results as well.
Date de publication: 2018-10-24
Rated 5 de
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Tsen par
Good Product 2
The antibody worked well at 1:500 dilution using mouse brain tissue extract.
Date de publication: 2018-08-29
Rated 2 de
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アセトン par
なんとも言えない
過剰発現したものには当たりましたが、内在性のものはdetectできませんでした。当たりが弱い気はしました。
Date de publication: 2018-07-03
Rated 2 de
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Kalevala par
not satisfied for IF
I tested this AB in IF (range 1/20 to 1/100) both in cells and brain E16 mouse
the signal was really low (PFA fixation in both case)
Date de publication: 2018-03-22
Rated 2 de
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Geroge par
no good signal in IF
I didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date de publication: 2018-03-07
Rated 3 de
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JDAlbarnaz par
It works for western blotting.
It detected a faint band with the expected size in nuclear extracts of immortalized HFFs, but not in total protein extracts (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date de publication: 2018-02-23