Anticorps CBP/KAT3A/CREBBP (C-1) recommandé pour la détection de CBP p265 d'origine mouse, rat, human et par WB, IP, IF, IHC(P) et ELISA

Anticorps CBP/KAT3A/CREBBP (C-1): sc-7300

Anticorps CBP/KAT3A/CREBBP (C-1) est évalué 4.3 de 5 de 37.
  • y_2022, m_8, d_13, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_37
  • loc_fr_FR, sid_7300, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 109ms
  • REVIEWS, PRODUCT
loading
5
20
4
12
3
1
2
3
1
1

Fiches techniques

    • Anti-L'anticorps CBP/KAT3A/CREBBP C-1 est un monoclonal IgG1 κ L'Anticorps CBP/KAT3A/CREBBP, cité dans 130 publications, fourni en 200 µg/ml
    • specific for an epitope mapping between amino acids 2423-2442 at the C-terminus of CBP of human origin (identical to corresponding mouse sequence)
    • L'Anticorps CBP/KAT3A/CREBBP (C-1) est recommandé pour la détection de CBP p265 d'origine mouse, rat, human et par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont et canine, bovine, porcine and avian
    • Anti-L'Anticorps CBP/KAT3A/CREBBP (C-1) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-7300 X, 200 µg/0.1 ml)
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CBP/KAT3A/CREBBP (C-1): sc-7300.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CBP/KAT3A/CREBBP Antibody (C-1). This work is in progress.
loading

Do you have a directly conjugated isotype control for this antibody. I bought sc-7300 AF594 and need a proper control to show that the positive signal is not a non-specific binding

Posée par: zhangll
Thank you for your question. The appropriate isotype control for AF594-conjugated sc-7300 is normal mouse IgG1 Alexa Fluor® 594: sc-516620.
Répondue par: Tech Support Europe
Date de publication: 2020-09-07

I recently purchased this antibody. However, I need to know if this antibody cross-reacts with the closely related p300 protein (KAT3B aka EP300) in human cells. A rapid and accurate response would be greatly appreciated. Thanks, Isaac

Posée par: IHilton
Thank you for your question. This mouse monoclonal antibody, CBP (C-1): sc-7300, is specific for CBP (CREB-binding protein). We have not specifically tested this clone for potential cross-reactivity with KAT3B aka EP300, however this antibody has been validated for specificity using our human siRNA for knockdown against the human gene target, CBP siRNA (h): sc-29244.
Répondue par: Technical Support
Date de publication: 2019-06-24

antibody Cat#sc-7300: how do I get in possession of the 10ug sample as advertised on your website? Thanks

Posée par: Seia
Thank you for your question. For a limited time, we are offering a free 10 µg sample of our mouse monoclonal antibody, CBP Antibody (C-1): sc-7300. Please contact our Technical Service Department and we would be happy to arrange a complimentary shipment for you. You may call us toll free at (800) 457-3801 option 2, or email [email protected], or use our live chat!
Répondue par: Technical Support
Date de publication: 2017-01-25

Is there any data showing this antibody is specific to CBP?

Posée par: jenni
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to CBP.
Répondue par: Technical Support 13
Date de publication: 2020-08-10
  • y_2022, m_8, d_13, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_fr_FR, sid_7300, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 123ms
  • QUESTIONS, PRODUCT
Rated 5 de 5 de par Looks visible and great This antibody works well with transfer buffer that included 0.1%SDS and only 10% methanol. Use for incubation at 1:500
Date de publication: 2018-12-12
Rated 5 de 5 de par Works in ChIP analysis In ChIP analysis, tThis antibody could detect forskolin-dependent binding of CBP to c-Fos promoter in HEK293FT cells.
Date de publication: 2018-10-29
Rated 1 de 5 de par not working for me in IFA I was unfortunately not successful in getting a signal with 96 well plate probed with recombinant CBP tagged protein O/N at 4C using a fluorescent secondary antibody for 1hr. Antibodies were concentrated as high as 1:50 in PBS-BSA and incubated for 1-2hrs at RT. I used an anti-CBP from a different vendor that gave me a specific signal. This was also tried on fixed cell lines expressing CBP on the surface with negative results as well.
Date de publication: 2018-10-24
Rated 5 de 5 de par Good Product 2 The antibody worked well at 1:500 dilution using mouse brain tissue extract.
Date de publication: 2018-08-29
Rated 2 de 5 de par なんとも言えない 過剰発現したものには当たりましたが、内在性のものはdetectできませんでした。当たりが弱い気はしました。
Date de publication: 2018-07-03
Rated 2 de 5 de par not satisfied for IF I tested this AB in IF (range 1/20 to 1/100) both in cells and brain E16 mouse the signal was really low (PFA fixation in both case)
Date de publication: 2018-03-22
Rated 2 de 5 de par no good signal in IF I didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date de publication: 2018-03-07
Rated 3 de 5 de par It works for western blotting. It detected a faint band with the expected size in nuclear extracts of immortalized HFFs, but not in total protein extracts (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date de publication: 2018-02-23
  • y_2022, m_8, d_13, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_37
  • loc_fr_FR, sid_7300, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT