Anticorps Cas9 (7A9-3A3)recommandé pour la détection de transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) d'origine Streptococcus pyogenes par WB, IP, IF, IHC(P) et FCM
Anti-L'anticorps Cas9 7A9-3A3 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps Cas9 fourni en 200 µg/ml
dirigé contre une protéine recombinante correspondant à la région N-terminal de Cas9 de Streptococcus pyogenes
L'Anticorps Cas9 (7A9-3A3) est recommandé pour la détection de transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) d'origine Streptococcus pyogenes par WB, IP, IF, IHC(P) et FCM
Anti-L'Anticorps Cas9 (7A9-3A3) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Cas9 (7A9-3A3): sc-517386.
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Cas9 Antibody (7A9-3A3) is a high quality monoclonal Cas9 antibody (also designated Cas9 antibody) suitable for the detection of the Cas9 protein of Streptococcus pyogenes origin. Cas9 Antibody (7A9-3A3) is available as both the non-conjugated anti-Cas9 antibody form, as well as multiple conjugated forms of anti-Cas9 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cas9 (CRISPR-associated endonuclease Cas9/Csn1) is a 1,368 amino acid RNA-guided endonuclease of Streptococcus pyogenes origin. Exclusively associated with the type II CRISPR locus, Cas9 nuclease consists of an RNA binding domain, an alpha helix recognition lobe (REC), a nuclease lobe that includes RuvC and HNH for DNA cleavage, and a protospacer adjacent motif (PAM) interacting site. Cas9 nuclease is involved in catalyzing site-specific double strand DNA cleavage. Characterized as being efficient in targeting genomic loci, Cas9 nuclease facilitates targeted genome editing. Cas9 nuclease along with short CRISPR RNAs (crRNAs) function as an RNA-guided endonuclease with RNA-directed target sequence recognition and protein-mediated DNA cleavage in the CRISPR/Cas system. The CRISPR/Cas system is an adaptive immune defense mechanism used by Archea and bacteria for the degradation of foreign genetic material, and has been manipulated to become a powerful tool in genomic engineering.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Répondue par: Tech Service
Date de publication: 2019-11-07
As the question above noted, what is the broad region of possible recognition by this antibody? (i.e. something like "AA1-200"). I plan to use this on a split-Cas9 and need to know whether it will be specific for the N-terminal piece.
Posée par: Nate C
Thank you for your question. This antibody was raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin. The exact binding epitope has not been mapped. It recognizes Cas9 variants including dCas9 (nuclease deficient Cas9). If you have any further questions, please contact Technical Service.
Répondue par: Tech Service
Date de publication: 2019-04-03
Is there any validation of this antibody working for immunofluorescence applications?
Posée par: diededh
Thank you for your question. Yes, this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has been validated to work for immunofluorescent staining applications. Unfortunately, we do not yet have any published IF data for this clone on our website. However, this clone, (7A9-3A3) has been widely published in the literature for use in a variety of applications, including IF. We do plan to update the data on our website once we have completed testing of the antibody.
Répondue par: Technical Support
Date de publication: 2019-03-13
What part of the N-terminus was this antibody raised against?
Posée par: Phil K
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has not yet been determined.
Rated 5 de
This antibody sample worked wellThis cas9 antibody worked well in HEK293T and Hela cells transfected with PX458 or PX459.
1:1000 2h at room temperature with 40ug cell lysate. I think the dilution ration can be much more than 1:1000.
Date de publication: 2018-09-25
Rated 5 de
Wubin Ma par
Great antibodyI got a sample and am so happy that work very well for western blotting. I use the ration 1:1500.
Date de publication: 2018-02-22
Rated 4 de
Date de publication: 2017-09-27
Rated 5 de
Works good.It shows a band at around 160 kDa, and some other bands, probably not specific, because I used high concentration of 1:300
Date de publication: 2017-08-11
Rated 5 de
successful resultI have used this antibody against cas9 ,got successful result. The antibody needs to be store at 4℃，if store at -20℃ it did not work.Very reliable,for dilution 1:400,good band seen.
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