Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Carbonyl reductase 3 CRISPR/Cas9 KO Plasmid (h): sc-406552
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Carbonyl reductase 3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Carbonyl reductase 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Carbonyl reductase 3 Antibody (E-12): sc-374393
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Carbonyl reductase 3 CRISPR/Cas9 KO Plasmid (h) | sc-406552 | 20 µg | $397.00 |
Overview
CBR3 encodes carbonyl reductase 3, a cytosolic NADPH-dependent oxidoreductase that catalyzes the reduction of reactive carbonyl compounds, including aldehydes and ketones generated during xenobiotic metabolism and oxidative stress. By modulating cellular redox balance and carbonyl detoxification, CBR3 contributes to phase I/II metabolic processes and helps limit accumulation of electrophilic intermediates that can damage proteins, lipids, and DNA. Variation in CBR3 activity has been studied in the context of differential handling of endogenous lipid peroxidation products and diverse small-molecule substrates, linking this enzyme to pathways relevant to stress responses and metabolic adaptation. Altered carbonyl reductase activity is frequently investigated in cancer biology, pharmacology, and toxicology models where redox state and metabolite processing influence cell survival and phenotype.
Carbonyl reductase 3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CBR3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CBR3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CBR3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Carbonyl reductase 3 protein expression.
This CRISPR knockout system enables efficient generation of CBR3-deficient cell models for investigation of Carbonyl reductase 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CBR3 exon(s) critical for Carbonyl reductase 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CBR3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Carbonyl reductase 3 CRISPR/Cas9 KO Plasmid (h) and Carbonyl reductase 3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CBR3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Carbonyl reductase 3 HDR Plasmid (h) and Carbonyl reductase 3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CBR3 homology arms to support homology-directed repair at defined CBR3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.